| RRC ID |
57509
|
| Author |
Shintani M, Yamane H, Nojiri H.
|
| Title |
Behavior of various hosts of the IncP-7 carbazole-degradative plasmid pCAR1 in artificial microcosms.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
pCAR1 endows carbazole-degrading ability on its host. Three different host Pseudomonas (P. fluorescens Pf0-1, P. resinovorans CA10dm4, and P. chlororaphis IAM1511) were prepared to compare their behaviors in artificial microcosms (sterile soil and water supplemented with carbazole) with previous results for P. putida SM1443 (Shintani et al., Appl. Microbiol. Biotechnol., 80, 485-497 (2008)). We monitored carbazole degradation, bacterial survival, and conjugative transfer of pCAR1. Carbazole degradations were not detected with any of three hosts in the soil microcosms, but were in most of the water microcosms. Degradative efficiency varied host by host. SM1443 is an appropriate host for carbazole degradation in soil microcosms, while CA10dm4 is for water microcosms. pCAR1 transfers were detected only in the water microcosms inoculated with SM1443 or IAM1511 as the host. This is the first report to compare the behaviors of various hosts of the same plasmid in these microcosms.
|
| Volume |
74(2)
|
| Pages |
343-9
|
| Published |
2010-1-1
|
| DOI |
10.1271/bbb.90675
|
| PII |
JST.JSTAGE/bbb/90675
|
| PMID |
20139612
|
| MeSH |
Biodegradation, Environmental
Carbazoles / metabolism*
Conjugation, Genetic
Plasmids*
Pseudomonas / genetics
Pseudomonas / metabolism
Soil / analysis*
Soil Microbiology
Soil Pollutants / metabolism*
Water Microbiology
Water Pollutants / metabolism*
|
| IF |
1.297
|
| Times Cited |
15
|
| Resource |
| General Microbes |
JCM 5832, JCM 5962, JCM 5967, JCM 2778, JCM 5963, JCM 13063 |
