論文 - 詳細
| RRC ID | 57510 |
|---|---|
| 著者 | Noguchi Y, Tanaka H, Kanamaru K, Kato M, Kobayashi T. |
| タイトル | Xylose triggers reversible phosphorylation of XlnR, the fungal transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus oryzae. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
XlnR is a transcription factor that mediates D-xylose-triggered induction of xylanolytic and cellulolytic genes in Aspergillus. In order to clarify the molecular mechanisms underlying XlnR-mediated induction, Aspergillus oryzae XlnR was fused with the c-myc tag and examined by Western blotting. Phosphate-affinity SDS-PAGE revealed that XlnR was present as a mixture of variously phosphorylated forms in the absence of D-xylose, and that D-xylose triggered additional phosphorylation of the protein. D-Xylose-triggered phosphorylation was a rapid process occurring within 5 min prior to the accumulation of xynG2 mRNA, and removal of D-xylose caused slow dephosphorylation, leading to less-phosphorylated forms. At 30 min after removal, the phosphorylation status was almost identical to that in the absence of D-xylose, and the level of xynG2 mRNA started to decrease. These results indicate that XlnR is highly phosphorylated when it is active in transactivation, implying that D-xylose-triggered reversible phosphorylation controls XlnR activity. |
| 巻・号 | 75(5) |
| ページ | 953-9 |
| 公開日 | 2011-1-1 |
| DOI | 10.1271/bbb.100923 |
| PII | JST.JSTAGE/bbb/100923 |
| PMID | 21597200 |
| MeSH | Aspergillus oryzae / drug effects Aspergillus oryzae / genetics* Aspergillus oryzae / metabolism Cellulose / metabolism* Fungal Proteins / genetics Fungal Proteins / metabolism* Glucose / pharmacology Phosphorylation / drug effects Proto-Oncogene Proteins c-myc / genetics Sugar Alcohols / pharmacology Trans-Activators / genetics Trans-Activators / metabolism* Transcription, Genetic / drug effects Transcriptional Activation* / drug effects Xylans / metabolism* Xylose / pharmacology* |
| IF | 1.297 |
| 引用数 | 32 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 16332 |