RRC ID 57510
Author Noguchi Y, Tanaka H, Kanamaru K, Kato M, Kobayashi T.
Title Xylose triggers reversible phosphorylation of XlnR, the fungal transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus oryzae.
Journal Biosci Biotechnol Biochem
Abstract XlnR is a transcription factor that mediates D-xylose-triggered induction of xylanolytic and cellulolytic genes in Aspergillus. In order to clarify the molecular mechanisms underlying XlnR-mediated induction, Aspergillus oryzae XlnR was fused with the c-myc tag and examined by Western blotting. Phosphate-affinity SDS-PAGE revealed that XlnR was present as a mixture of variously phosphorylated forms in the absence of D-xylose, and that D-xylose triggered additional phosphorylation of the protein. D-Xylose-triggered phosphorylation was a rapid process occurring within 5 min prior to the accumulation of xynG2 mRNA, and removal of D-xylose caused slow dephosphorylation, leading to less-phosphorylated forms. At 30 min after removal, the phosphorylation status was almost identical to that in the absence of D-xylose, and the level of xynG2 mRNA started to decrease. These results indicate that XlnR is highly phosphorylated when it is active in transactivation, implying that D-xylose-triggered reversible phosphorylation controls XlnR activity.
Volume 75(5)
Pages 953-9
Published 2011-1-1
DOI 10.1271/bbb.100923
PII JST.JSTAGE/bbb/100923
PMID 21597200
MeSH Aspergillus oryzae / drug effects Aspergillus oryzae / genetics* Aspergillus oryzae / metabolism Cellulose / metabolism* Fungal Proteins / genetics Fungal Proteins / metabolism* Glucose / pharmacology Phosphorylation / drug effects Proto-Oncogene Proteins c-myc / genetics Sugar Alcohols / pharmacology Trans-Activators / genetics Trans-Activators / metabolism* Transcription, Genetic / drug effects Transcriptional Activation* / drug effects Xylans / metabolism* Xylose / pharmacology*
IF 1.297
Times Cited 32
General Microbes JCM 16332