| RRC ID |
57516
|
| Author |
Yamauchi N, Tanoue R.
|
| Title |
Deuterium incorporation experiments from (3R)- and (3S)-[3-2H]leucine into characteristic isoprenoidal lipid-core of halophilic archaea suggests the involvement of isovaleryl-CoA dehydrogenase.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
The stereochemical reaction course for the two C-3 hydrogens of leucine to produce a characteristic isoprenoidal lipid in halophilic archaea was observed using incubation experiments with whole cell Halobacterium salinarum. Deuterium-labeled (3R)- and (3S)-[3-2H]leucine were freshly prepared as substrates from 2,3-epoxy-4-methyl-1-pentanol. Incorporation of deuterium from (3S)-[3-2H]leucine and loss of deuterium from (3R)-[3-2H]leucine in the lipid-core of H. salinarum was observed. Taken together with the results of our previous report, involving the incubation of chiral-labeled [5-2H]leucine, these results strongly suggested an involvement of isovaleryl-CoA dehydrogenase in leucine conversion to isoprenoid lipid in halophilic archaea. The stereochemical course of the reaction (anti-elimination) might have been the same as that previously reported for mammalian enzyme reactions. Thus, these results suggested that branched amino acids were metabolized to mevalonate in archaea in a manner similar to other organisms.
|
| Volume |
81(11)
|
| Pages |
2062-2070
|
| Published |
2017-11-1
|
| DOI |
10.1080/09168451.2017.1373588
|
| PMID |
28942710
|
| MeSH |
Deuterium / chemistry*
Halobacterium salinarum / metabolism*
Isovaleryl-CoA Dehydrogenase / metabolism*
Leucine / chemistry*
Lipid Metabolism
Lipids / chemistry*
Terpenes / chemistry*
|
| IF |
1.297
|
| Times Cited |
1
|
| Resource |
| General Microbes |
JCM 9210 |
