Reference - Detail
| RRC ID | 57516 |
|---|---|
| Author | Yamauchi N, Tanoue R. |
| Title | Deuterium incorporation experiments from (3R)- and (3S)-[3-2H]leucine into characteristic isoprenoidal lipid-core of halophilic archaea suggests the involvement of isovaleryl-CoA dehydrogenase. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
The stereochemical reaction course for the two C-3 hydrogens of leucine to produce a characteristic isoprenoidal lipid in halophilic archaea was observed using incubation experiments with whole cell Halobacterium salinarum. Deuterium-labeled (3R)- and (3S)-[3-2H]leucine were freshly prepared as substrates from 2,3-epoxy-4-methyl-1-pentanol. Incorporation of deuterium from (3S)-[3-2H]leucine and loss of deuterium from (3R)-[3-2H]leucine in the lipid-core of H. salinarum was observed. Taken together with the results of our previous report, involving the incubation of chiral-labeled [5-2H]leucine, these results strongly suggested an involvement of isovaleryl-CoA dehydrogenase in leucine conversion to isoprenoid lipid in halophilic archaea. The stereochemical course of the reaction (anti-elimination) might have been the same as that previously reported for mammalian enzyme reactions. Thus, these results suggested that branched amino acids were metabolized to mevalonate in archaea in a manner similar to other organisms. |
| Volume | 81(11) |
| Pages | 2062-2070 |
| Published | 2017-11-1 |
| DOI | 10.1080/09168451.2017.1373588 |
| PMID | 28942710 |
| MeSH | Deuterium / chemistry* Halobacterium salinarum / metabolism* Isovaleryl-CoA Dehydrogenase / metabolism* Leucine / chemistry* Lipid Metabolism Lipids / chemistry* Terpenes / chemistry* |
| IF | 1.297 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 9210 |