論文 - 詳細
| RRC ID | 57539 |
|---|---|
| 著者 | Herpin A, Schmidt C, Kneitz S, Gobé C, Regensburger M, Le Cam A, Montfort J, Adolfi MC, Lillesaar C, Wilhelm D, Kraeussling M, Mourot B, Porcon B, Pannetier M, Pailhoux E, Ettwiller L, Dolle D, Guiguen Y, Schartl M. |
| タイトル | A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka. |
| ジャーナル | PLoS Biol |
| Abstract |
Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1-acting as master sex-determining gene-has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3' UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans-together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells-suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates. |
| 巻・号 | 17(4) |
| ページ | e3000185 |
| 公開日 | 2019-4-1 |
| DOI | 10.1371/journal.pbio.3000185 |
| PII | PBIOLOGY-D-18-00818 |
| PMID | 30947255 |
| PMC | PMC6448818 |
| MeSH | 3' Untranslated Regions / genetics Animals Biological Evolution Female Fish Proteins / genetics Gene Expression Regulation, Developmental / genetics* Germ Cells / metabolism Male Oryzias / genetics* RNA Recognition Motif Proteins / metabolism RNA Stability / genetics RNA, Messenger / metabolism Sex Determination Processes / genetics* Transcription Factors / genetics Transcription Factors / metabolism Vertebrates / metabolism |
| IF | 8.386 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | BAC clones |