Reference - Detail
| RRC ID | 57624 |
|---|---|
| Author | Hasan I, Gerdol M, Fujii Y, Ozeki Y. |
| Title | Functional Characterization of OXYL, A SghC1qDC LacNAc-specific Lectin from The Crinoid Feather Star Anneissia Japonica. |
| Journal | Mar Drugs |
| Abstract |
We identified a lectin (carbohydrate-binding protein) belonging to the complement 1q(C1q) family in the feather star Anneissia japonica (a crinoid pertaining to the phylum Echinodermata). The combination of Edman degradation and bioinformatics sequence analysis characterized the primary structure of this novel lectin, named OXYL, as a secreted 158 amino acid-long globular head (sgh)C1q domain containing (C1qDC) protein. Comparative genomics analyses revealed that OXYL pertains to a family of intronless genes found with several paralogous copies in different crinoid species. Immunohistochemistry assays identified the tissues surrounding coelomic cavities and the arms as the main sites of production of OXYL. Glycan array confirmed that this lectin could quantitatively bind to type-2 N-acetyllactosamine (LacNAc: Galβ1-4GlcNAc), but not to type-1 LacNAc (Galβ1-3GlcNAc). Although OXYL displayed agglutinating activity towards Pseudomonas aeruginosa, it had no effect on bacterial growth. On the other hand, it showed a significant anti-biofilm activity. We provide evidence that OXYL can adhere to the surface of human cancer cell lines BT-474, MCF-7, and T47D, with no cytotoxic effect. In BT-474 cells, OXYL led to a moderate activation of the p38 kinase in the MAPK signaling pathway, without affecting the activity of caspase-3. Bacterial agglutination, anti-biofilm activity, cell adhesion, and p38 activation were all suppressed by co-presence of LacNAc. This is the first report on a type-2 LacNAc-specific lectin characterized by a C1q structural fold. |
| Volume | 17(2) |
| Published | 2019-2-25 |
| DOI | 10.3390/md17020136 |
| PII | md17020136 |
| PMID | 30823584 |
| PMC | PMC6409975 |
| MeSH | Agglutination / drug effects Amino Acid Sequence Amino Sugars / chemistry Amino Sugars / metabolism Animals Base Sequence Biofilms / drug effects Caspase 3 / metabolism Cell Line, Tumor Echinodermata / chemistry* Humans Lectins / chemistry Lectins / genetics Lectins / metabolism Lectins / pharmacology* Protein Binding Pseudomonas aeruginosa / drug effects p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 4.073 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.intestinalis / (O.japonicus) | |