| RRC ID |
57626
|
| 著者 |
Otaki R, Oishi Y, Abe S, Fujiwara S, Sato N.
|
| タイトル |
Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri.
|
| ジャーナル |
Bioresour Technol
|
| Abstract |
Chlorella kessleri accumulates triacylglycerol usable for biodiesel-fuel production to >20% dry cell weight in three days when cultured in three-fold diluted seawater, which imposes the combinatory stress of hyperosmosis and nutrients limitation. The quantitative behavior of major C-compounds, and related-gene expression patterns were investigated in Chlorella cells stressed with hyperosmosis, nutrients limitation, or their combination, to elucidate the C-metabolism for economical seawater-based triacylglycerol accumulation. Combinatory-stress cells showed repressed protein synthesis with initially accumulated starch being degraded later, the C-metabolic flow thereby being diverted to fatty acid and subsequent triacylglycerol accumulation. This C-flow diversion was induced by cooperative actions of nutrients-limitation and hyperosmosis. Semi-quantitative PCR analysis implied positive rewiring of the diverted C-flow into triacylglycerol in combinatory-stress cells through upregulation of gene expression concerning fatty acid and triacylglycerol synthesis, and starch synthesis and degradation. The information of regulatory C-metabolism will help reinforce the seawater-based triacylglycerol accumulation ability in algae including Chlorella.
|
| 巻・号 |
289
|
| ページ |
121686
|
| 公開日 |
2019-10-1
|
| DOI |
10.1016/j.biortech.2019.121686
|
| PII |
S0960-8524(19)30916-2
|
| PMID |
31238290
|
| MeSH |
Biofuels
Carbon / metabolism*
Chlorella / metabolism*
Fatty Acids / metabolism
Seawater
Starch / metabolism
Triglycerides / biosynthesis*
|
| IF |
7.539
|
| 引用数 |
0
|
| リソース情報 |
| 藻類 |
NIES-2152 |
