RRC ID 57643
Author Fukunaga H, Kaminaga K, Sato T, Butterworth KT, Watanabe R, Usami N, Ogawa T, Yokoya A, Prise KM.
Title High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation.
Journal Sci Rep
Abstract Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one century ago, the TSE of MRT in the testes and its clinical importance for preventing male fertility remain to be determined. In this study, using the combination of MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-mediated TSE for the preservation of spermatogenesis. Furthermore, our high-precision microbeam analysis revealed that the survival and potential migration steps of the non-irradiated germ stem cells in the irradiated testes tissue would be needed for the effective TSE for spermatogenesis. Our findings indicated the distribution of dose irradiated in the testes at the microscale level is of clinical importance for delivering high doses of radiation to the tumor, while still preserving male fertility.
Volume 9(1)
Pages 12618
Published 2019-10-1
DOI 10.1038/s41598-019-48772-3
PII 10.1038/s41598-019-48772-3
PMID 31575926
PMC PMC6773706
MeSH Animals Cell Movement / radiation effects* Cell Survival / radiation effects Fertility Preservation* Germ Cells* / metabolism Germ Cells* / pathology Male Mice Mice, Transgenic Spermatogenesis / radiation effects* Testis* / metabolism Testis* / pathology X-Ray Therapy*
IF 3.998
Times Cited 3
Mice RBRC00445