論文 - 詳細
| RRC ID | 57661 |
|---|---|
| 著者 | Sekiguchi T, Nohiro Y, Nakamura Y, Hisamoto N, Nishimoto T. |
| タイトル | The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. |
| ジャーナル | Mol Cell Biol |
| Abstract |
The human CCG1 gene complements tsBN462, a temperature-sensitive G1 mutant of the BHK21 cell line. The previously cloned cDNA turned out to be a truncated form of the actual CCG1 cDNA. The newly cloned CCG1 cDNA was 6.0 kb and encoded a protein with a molecular mass of 210 kDa. Using an antibody to a predicted peptide from the CCG1 protein, a protein with a molecular mass of over 200 kDa was identified in human, monkey, and hamster cell lines. In the newly defined C-terminal region, an acidic domain was found. It contained four consensus target sequences for casein kinase II and was phosphorylated by this enzyme in vitro. However, this C-terminal region was not required to complement tsBN462 mutation since the region encoding the C-terminal part was frequently missing in complemented clones derived by DNA-mediated gene transfer. CCG1 contains a sequence similar to the putative DNA-binding domain of HMG1 in addition to the previously detected amino acid sequences common in nuclear proteins, such as a proline cluster and a nuclear translocation signal. Consistent with these predictions, CCG1 was present in nuclei, possessed DNA-binding activity, and was eluted with similar concentrations of salt, 0.3 to 0.4 M NaCl either from isolated nuclei or from a DNA-cellulose column. |
| 巻・号 | 11(6) |
| ページ | 3317-25 |
| 公開日 | 1991-6-1 |
| DOI | 10.1128/mcb.11.6.3317-3325.1991 |
| PMID | 2038334 |
| PMC | PMC360184 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Codon / genetics DNA / genetics DNA / isolation & purification DNA-Binding Proteins / genetics* DNA-Binding Proteins / isolation & purification DNA-Binding Proteins / metabolism Escherichia coli / genetics G1 Phase* Histone Acetyltransferases Humans Molecular Sequence Data Molecular Weight Nuclear Proteins / genetics* Nuclear Proteins / isolation & purification Nuclear Proteins / metabolism Oligonucleotide Probes Polymerase Chain Reaction Recombinant Proteins / isolation & purification Sequence Homology, Nucleic Acid TATA-Binding Protein Associated Factors* Temperature Transcription Factor TFIID* |
| IF | 3.611 |
| 引用数 | 123 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBSCCG1 (RDB01394) |