RRC ID 57661
Author Sekiguchi T, Nohiro Y, Nakamura Y, Hisamoto N, Nishimoto T.
Title The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.
Journal Mol Cell Biol
Abstract The human CCG1 gene complements tsBN462, a temperature-sensitive G1 mutant of the BHK21 cell line. The previously cloned cDNA turned out to be a truncated form of the actual CCG1 cDNA. The newly cloned CCG1 cDNA was 6.0 kb and encoded a protein with a molecular mass of 210 kDa. Using an antibody to a predicted peptide from the CCG1 protein, a protein with a molecular mass of over 200 kDa was identified in human, monkey, and hamster cell lines. In the newly defined C-terminal region, an acidic domain was found. It contained four consensus target sequences for casein kinase II and was phosphorylated by this enzyme in vitro. However, this C-terminal region was not required to complement tsBN462 mutation since the region encoding the C-terminal part was frequently missing in complemented clones derived by DNA-mediated gene transfer. CCG1 contains a sequence similar to the putative DNA-binding domain of HMG1 in addition to the previously detected amino acid sequences common in nuclear proteins, such as a proline cluster and a nuclear translocation signal. Consistent with these predictions, CCG1 was present in nuclei, possessed DNA-binding activity, and was eluted with similar concentrations of salt, 0.3 to 0.4 M NaCl either from isolated nuclei or from a DNA-cellulose column.
Volume 11(6)
Pages 3317-25
Published 1991-6
DOI 10.1128/mcb.11.6.3317
PMID 2038334
PMC PMC360184
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Codon / genetics DNA / genetics DNA / isolation & purification DNA-Binding Proteins / genetics* DNA-Binding Proteins / isolation & purification DNA-Binding Proteins / metabolism Escherichia coli / genetics G1 Phase* Histone Acetyltransferases Humans Molecular Sequence Data Molecular Weight Nuclear Proteins / genetics* Nuclear Proteins / isolation & purification Nuclear Proteins / metabolism Oligonucleotide Probes Polymerase Chain Reaction Recombinant Proteins / isolation & purification Sequence Homology, Nucleic Acid TATA-Binding Protein Associated Factors* Temperature Transcription Factor TFIID*
IF 3.735
Times Cited 123
Resource
DNA material pBSCCG1 (RDB01394)