論文 - 詳細
| RRC ID | 57728 |
|---|---|
| 著者 | Muramoto T, Iriki H, Watanabe J, Kawata T. |
| タイトル | Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium. |
| ジャーナル | Cells |
| Abstract |
In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2⁻3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium. |
| 巻・号 | 8(1) |
| 公開日 | 2019-1-12 |
| DOI | 10.3390/cells8010046 |
| PII | cells8010046 |
| PMID | 30642074 |
| PMC | PMC6356401 |
| MeSH | CRISPR-Associated Protein 9 / genetics* CRISPR-Cas Systems* Dictyostelium / genetics* Gene Editing / trends* Genetic Vectors / genetics* Homologous Recombination |
| IF | 5.656 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | G90483 |