論文 - 詳細
| RRC ID | 57732 |
|---|---|
| 著者 | Narita Y, Shimizu K, Ikemoto K, Uchino R, Kosugi M, Maess MB, Magata Y, Oku N, Ogawa M. |
| タイトル | Macrophage-targeted, enzyme-triggered fluorescence switch-on system for detection of embolism-vulnerable atherosclerotic plaques. |
| ジャーナル | J Control Release |
| Abstract |
The development of atherosclerotic plaques is a critical step that can result in an arterial embolism. Therefore, detection of these vulnerable plaques is of clinical significance for the diagnosis of atherosclerosis. However, there are few imaging systems able to detect such plaques easily. In this study, we designed a new platform for near-infrared fluorescence (NIRF) imaging of macrophages in atherosclerotic plaques, one using both a liposomal DDS and an activatable fluorescent probe, and evaluated the utility of this imaging for the diagnosis of atherosclerosis. We first synthesized a fluorescent switch-on probe, Peptide-ICG2, which is optically silent under normal conditions but activated in the presence of the lysosomal enzyme, cathepsin B. To achieve macrophage-specific fluorescence activation, we encapsulated Peptide-ICG2 into phosphatidylserine-containing liposome (P-ICG2-PS-Lip), since the accumulation of phosphatidylserine receptor-bearing macrophages is characteristic of embolism-vulnerable plaques. The experiments using macrophage-like RAW264 cells in culture showed that P-ICG2-PS-Lip was selectively taken up into the cells and that significant fluorescence of the probe was observed. For NIRF imaging of the atherosclerotic plaques, P-ICG2-PS-Lip was intravenously injected into ApoE-knockout atherosclerotic model mice or WHHL rabbits, and the fluorescence at the aortae was imaged. The results indicated that ICG fluorescence could be successfully observed at the plaques on the artery walls. The results of the present study thus suggest that NIRF imaging using P-ICG2-PS-Lip would be useful for detecting embolism-vulnerable atherosclerotic plaques. |
| 巻・号 | 302 |
| ページ | 105-115 |
| 公開日 | 2019-5-28 |
| DOI | 10.1016/j.jconrel.2019.03.025 |
| PII | S0168-3659(19)30183-X |
| PMID | 30936020 |
| MeSH | Animals Aorta / metabolism Cathepsin B / metabolism* Cell Membrane Permeability Embolism / physiopathology Female Fluorescent Dyes / chemistry* Indocyanine Green / chemistry* Liposomes / chemistry Macrophages / metabolism* Mice Mice, Knockout, ApoE Optical Imaging Peptides / chemistry Phosphatidylserines / chemistry Plaque, Atherosclerotic / diagnostic imaging* RAW 264.7 Cells Rabbits Spectrometry, Fluorescence Spectroscopy, Near-Infrared |
| IF | 7.727 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |