| RRC ID |
57739
|
| 著者 |
Harada Y, Kizuka Y, Tokoro Y, Kondo K, Yagi H, Kato K, Inoue H, Taniguchi N, Maruyama I.
|
| タイトル |
N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
We investigated the correlation between metastatic behaviors of tumor cells and asparagine-linked glycosylation (N-glycosylation) of tumor-derived extracellular vesicles (EVs). Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N-glycosylation. All melanoma variants and EVs have nearly identical profiles of de-sialylated N-glycans. The major de-sialylated N-glycan structures of cells and EVs are core-fucosylated, tetra-antennary N-glycans with β1,6-N-acetylglucosamine branches. A few N-glycans are extended by N-acetyllactosamine repeats. Sialylation of these N-glycans may generate cell-type-specific N-glycomes on EVs. Taken together, melanoma-derived EVs show high expression of tumor-associated N-glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs.
|
| 巻・号 |
593(9)
|
| ページ |
942-951
|
| 公開日 |
2019-5-1
|
| DOI |
10.1002/1873-3468.13377
|
| PMID |
30943309
|
| PMC |
PMC6594130
|
| MeSH |
Animals
Extracellular Vesicles / metabolism*
Glycosylation
Glycosyltransferases / metabolism
Melanoma, Experimental / pathology*
Mice
Nitrogen / metabolism*
|
| IF |
3.057
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
B16/BL6(RCB2638) |
