論文 - 詳細
| RRC ID | 57744 |
|---|---|
| 著者 | Zhelev Z, Georgieva E, Lazarova D, Semkova S, Aoki I, Gulubova M, Higashi T, Bakalova R. |
| タイトル | "Redox Imaging" to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers. |
| ジャーナル | Oxid Med Cell Longev |
| Abstract |
The present study was directed to the development of EPR methodology for distinguishing cells with different proliferative activities, using "redox imaging." Three nitroxide radicals were used as redox sensors: (a) mito-TEMPO-cell-penetrating and localized mainly in the mitochondria; (b) methoxy-TEMPO-cell-penetrating and randomly distributed between the cytoplasm and the intracellular organelles; and (c) carboxy-PROXYL-nonpenetrating in living cells and evenly distributed in the extracellular environment. The experiments were conducted on eleven cell lines with different proliferative activities and oxidative capacities, confirmed by conventional analytical tests. The data suggest that cancer cells and noncancer cells are characterized by a completely different redox status. This can be analyzed by EPR spectroscopy using mito-TEMPO and methoxy-TEMPO, but not carboxy-PROXYL. The correlation analysis shows that the EPR signal intensity of mito-TEMPO in cell suspensions is closely related to the superoxide level. The described methodology allows the detection of overproduction of superoxide in living cells and their identification based on the intracellular redox status. The experimental data provide evidences about the role of superoxide and hydroperoxides in cell proliferation and malignancy. |
| 巻・号 | 2019 |
| ページ | 6373685 |
| 公開日 | 2019-1-1 |
| DOI | 10.1155/2019/6373685 |
| PMID | 31089411 |
| PMC | PMC6476105 |
| MeSH | Antioxidants / metabolism Biomarkers / metabolism* Cell Line Cell Proliferation Cyclic N-Oxides / metabolism Electron Spin Resonance Spectroscopy Humans Hydrogen Peroxide / metabolism* Leukemia / metabolism Lymphocytes / metabolism Nitrogen Oxides / chemistry Nitrogen Oxides / metabolism Oxidation-Reduction Superoxides / metabolism* |
| IF | 5.076 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |