RRC ID 57765
著者 Uda RM, Nishimoto N, Matsui T, Takagi S.
タイトル Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA.
ジャーナル Soft Matter
Abstract Designing ligands that selectively target G-quadruplex DNAs has gained attention due to their possible roles in regulation of gene expression and as anti-cancer agents. In this article, we report irradiation-induced ligand binding to G-quadruplex DNAs which offers a novel approach to targeting specific G-quadruplexes. Photoinduced binding to G-quadruplex DNAs was observed for copolymers of poly(vinyl alcohol) carrying a malachite green moiety (PVAMG). This molecule has an aromatic ring with cationic charge, which after irradiation becomes a binding site for G-quadruplex DNA. PVAMGs acted as neutral polymers with no binding affinity under dark conditions. The photoinduced binding was revealed by fluorescence spectroscopy, NMR spectroscopy, UV melting curve, and DNA polymerase stop assay. PVAMGs showed preference to parallel G-quadruplex structures over mixed parallel/antiparallel structures. PVAMGs were found to be noncytotoxic under both dark and irradiated conditions up to a concentration of 20 μM.
巻・号 15(22)
ページ 4454-4459
公開日 2019-6-5
DOI 10.1039/c9sm00411d
PMID 31073583
MeSH G-Quadruplexes* Polymers / chemistry* Polymers / radiation effects Polyvinyl Alcohol / chemistry Rosaniline Dyes / chemistry* Rosaniline Dyes / radiation effects Ultraviolet Rays
IF 3.399
引用数 0
リソース情報
ヒト・動物細胞 Colon-26(RCB2657)