| RRC ID |
57772
|
| 著者 |
Miura S, Kobune M, Horiguchi H, Kikuchi S, Iyama S, Murase K, Goto A, Ikeda H, Takada K, Miyanishi K, Kato J.
|
| タイトル |
EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cells.
|
| ジャーナル |
Blood Cells Mol Dis
|
| Abstract |
Recently, a new erythroid regulator, erythroferrone (ERFE), which downregulates hepatic hepcidin production, has been identified. However, the relationship between ERFE and abnormal iron metabolism in MDS is unclear. In this study, we examined the level of ERFE mRNA during ex vivo erythroid differentiation using cord blood CD34+ cells and we further analyzed whether ERFE could be produced by MDS cells using a public database (GSE58831). ERFE mRNA was increased during normal erythroid differentiation. An analysis of GSE58831 indicated that ERFE expression in bone marrow (BM) MDS cells was higher than that in healthy volunteer (HV)-derived BM cells. ERFE expression significantly and positively correlated with the expression of erythropoietin (EPO) receptors (EPO-R), ALAS2 (5'-Aminolevulinate Synthase 2), STEAP3 (STEAP family member 3) and the presence of ring sideroblasts or the SF3B1 mutation. These results suggest that EPO-R+ MDS cells with ring sideroblasts or an SF3B1 mutation produce high levels of ERFE that may be associated with a reduction in hepcidin.
|
| 巻・号 |
78
|
| ページ |
1-8
|
| 公開日 |
2019-9-1
|
| DOI |
10.1016/j.bcmd.2019.04.014
|
| PII |
S1079-9796(19)30133-0
|
| PMID |
31082798
|
| MeSH |
Alleles
Biomarkers
Blood Transfusion
Cell Differentiation
Cell Line
Erythroblasts / metabolism*
Erythroblasts / pathology
Erythroid Precursor Cells / metabolism
Ferritins / blood
Gene Expression Regulation*
Hepatocytes / metabolism*
Hepcidins / genetics*
Humans
Immunophenotyping
Myelodysplastic Syndromes / etiology
Myelodysplastic Syndromes / metabolism*
Myelodysplastic Syndromes / pathology
Myelodysplastic Syndromes / therapy
Peptide Hormones / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Erythropoietin / metabolism*
|
| IF |
2.46
|
| 引用数 |
3
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
