論文 - 詳細
| RRC ID | 57778 |
|---|---|
| 著者 | Oki Y, Kirita K, Ohta S, Ohba S, Horiguchi I, Sakai Y, Ito T. |
| タイトル | Switching of Cell Proliferation/Differentiation in Thiol-Maleimide Clickable Microcapsules Triggered by in Situ Conjugation of Biomimetic Peptides. |
| ジャーナル | Biomacromolecules |
| Abstract |
Extracellular environments significantly affect cell proliferation, differentiation, and functions. The extracellular environment changes during many physiological and pathological processes such as embryo development, wound healing, and tumor growth. To mimic these changes, we developed novel thiol-maleimide clickable alginate microcapsules, which can introduce thiol-containing peptides by " in situ conjugation" with maleimide-modified alginate, even in serum-containing cell culture media. Additive peptides were rapidly concentrated into microcapsules by a diffusion-reaction process in the capsule. The proliferation of encapsulated fibroblasts was accelerated by in situ conjugation of CRGDS, while free RGDS showed no effect. Moreover, encapsulated preosteoblastic cells started osteogenic differentiation via in situ conjugation of BMP-2 mimetic peptides such as CDWIVA and CG-BMP-2 knuckle epitope peptide, while BMP-2 did not induce differentiation of the encapsulated cells. Especially in tissue engineering, accurate and inexpensive methods for inducing cell differentiation are required. We believe that this in situ conjugation approach employing various functional peptides will be useful in biomedical, bioindustrial, and biochemical fields in the future. |
| 巻・号 | 20(6) |
| ページ | 2350-2359 |
| 公開日 | 2019-6-10 |
| DOI | 10.1021/acs.biomac.9b00333 |
| PMID | 31059241 |
| MeSH | Animals Biomimetic Materials* / chemical synthesis Biomimetic Materials* / chemistry Biomimetic Materials* / pharmacology Capsules Cell Differentiation / drug effects* Cell Proliferation / drug effects* Cells, Immobilized / cytology Cells, Immobilized / metabolism* Click Chemistry* Maleimides / chemistry Mice NIH 3T3 Cells Sulfhydryl Compounds / chemistry |
| IF | 6.092 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH/3T3(RCB2767) MC3T3-E1(RCB1126) |