| RRC ID |
57802
|
| Author |
Shimizu Y, Sakuragi N, Nakamura K, Taira T, Ayabe T, Fukui A.
|
| Title |
A simple culture method for liver and intestinal tissue-resident macrophages from neonatal mice.
|
| Journal |
In Vitro Cell Dev Biol Anim
|
| Abstract |
The liver and intestine contain a remarkably large portion of tissue-resident macrophage cells representing a phenotype that downregulates inflammation and initiates tissue repair. Here, liver and intestinal tissues obtained from neonatal mice were minced, enzymatically digested, and incubated in RPMI1640-based media. In a 2-wk culture, spherical floating cells emerged on a fibroblastic sheet. These cells showed phagocytic activity and F4/80+-CD11b+-CD206+-Arg1+-iNOS--CD209a- phenotype, suggesting that these cells are tissue-resident macrophages. These macrophages proliferated in the co-culture system in the presence of fibroblastic feeder cell layer and absence of supplemental cytokines; the co-culture system did not cause a significant change in the phenotype of cells grown in a 4-wk culture. On the feeder cells, macrophage density was approximately 1.5 × 104/cm2 and the doubling time was approximately 70 h. Based on these observations, we present a simple method for the isolation and propagation of tissue-resident macrophages resembling M2 macrophage from neonatal mice, and this method provides a useful platform for in vitro studies of tissue-resident macrophages.
|
| Volume |
55(6)
|
| Pages |
436-444
|
| Published |
2019-6-1
|
| DOI |
10.1007/s11626-019-00359-y
|
| PII |
10.1007/s11626-019-00359-y
|
| PMID |
31119642
|
| MeSH |
Animals
Animals, Newborn
Cell Culture Techniques / methods*
Cell Separation / methods*
Coculture Techniques
Female
Fibroblasts / cytology
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression Regulation
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Intestine, Small / cytology*
Liver / cytology*
Macrophages / cytology*
Macrophages / physiology
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Transgenic
Phagocytosis
|
| IF |
1.665
|
| Times Cited |
0
|
| Resource |
| Human and Animal Cells |
NIH3T3-3-4(RCB1862) |
