RRC ID 57806
Author Nakayama H, Ohuchida K, Yonenaga A, Sagara A, Ando Y, Kibe S, Takesue S, Abe T, Endo S, Koikawa K, Okumura T, Shido K, Miyoshi K, Nakata K, Moriyama T, Miyasaka Y, Inoue S, Ohtsuka T, Mizumoto K, Nakamura M.
Title S100P regulates the collective invasion of pancreatic cancer cells into the lymphatic endothelial monolayer.
Journal Int J Oncol
Abstract Lymph node metastasis is an independent prognostic factor in pancreatic cancer. However, the mechanisms of lymph node colonization are unknown. As a mechanism of lymphatic metastasis, it has been reported for other types of cancer that spheroids from tumor cells cause circular chemorepellent‑induced defects (CCIDs) in lymphatic endothelial monolayers. In pancreatic cancer, such mechanisms of metastasis have not been elucidated. The present study evaluated the involvement of this new mechanism of metastasis in pancreatic cancer and investigated the associated factors. In human pancreatic cancer tissue, it was observed that clusters of cancer cells penetrated the wall of lymphatic ducts around the primary tumor. An in vitro co‑culture system was then used to analyze the mechanisms of tumor cell‑mediated disruption of lymphatic vessels. Time‑lapse microscopic imaging revealed that spheroids from pancreatic cancer cells caused circular defects in lymphatic endothelial monolayers. CCID formation ability differed depending on the cell line. Neither aggregation of spheroids nor adhesion to lymphatic endothelial cells (LECs) exhibited a significant correlation with this phenomenon. The addition of supernatant from cultured cancer cells enhanced CCID formation. Microarray analysis revealed that the expression of S100 calcium binding protein P (S100P) was significantly increased when LECs were treated with supernatant from cultured cancer cells. Addition of a S100P antagonist significantly suppressed the migration of LECs and CCID formation. The present findings demonstrated that spheroids from pancreatic cancer cells caused circular defects in lymphatic endothelial monolayers. These CCIDs in pancreatic cancer were partly regulated by S100P, suggesting that S100P may be a promising target to inhibit lymph node metastasis.
Volume 55(1)
Pages 211-222
Published 2019-7
DOI 10.3892/ijo.2019.4812
PMID 31180531
MeSH Adult Aged Aged, 80 and over Animals Antigens, Nuclear / metabolism* Autoantigens / metabolism* Cell Adhesion / physiology Cell Line, Tumor Endothelial Cells / metabolism Endothelial Cells / pathology* Female Humans Immunohistochemistry Lymph Nodes / metabolism Lymph Nodes / pathology Lymphatic Metastasis Male Mice Middle Aged Neoplasm Invasiveness Pancreatic Neoplasms / metabolism Pancreatic Neoplasms / pathology* Spheroids, Cellular
IF 3.571
Times Cited 1
Resource
Human and Animal Cells PANC-1(RCB2095)