RRC ID 57839
著者 Miyazaki Y, Iwaihara Y, Bak J, Nakano H, Takeuchi S, Takeuchi H, Matsui T, Tachikawa D.
タイトル The cooperative induction of macrophage activation by fucoidan derived from Cladosiphon okamuranus and β-glucan derived from Saccharomyces cerevisiae.
ジャーナル Biochem Biophys Res Commun
Abstract The present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived β-glucan. The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in RAW264 cells were remarkably enhanced in the presence of 10 μg/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-β-cyclodextrin (MβCD), a general agent for disruption of lipid rafts, diminished that. Furthermore, it was revealed that the additive effects of zymosan on the immune activation with fucoidan was thought to be mediated by dectin-1 based on the results with dectin-1-knockdown RAW264 cells. All of results suggested that fucoidan and some kinds of β-glucan would cooperatively reinforce the activity of innate immune cells via interactive receptor crosstalk.
巻・号 516(1)
ページ 245-250
公開日 2019-8-13
DOI 10.1016/j.bbrc.2019.06.010
PII S0006-291X(19)31120-9
PMID 31221482
MeSH Animals Drug Synergism Macrophage Activation / drug effects* Macrophages / drug effects* Macrophages / immunology Mice Phaeophyta / chemistry Polysaccharides / chemistry Polysaccharides / pharmacology* RAW 264.7 Cells Saccharomyces cerevisiae / chemistry Seaweed / chemistry beta-Glucans / chemistry beta-Glucans / pharmacology*
IF 2.985
引用数 0
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)