RRC ID 57842
著者 Kusuyama J, Seong C, Makarewicz NS, Ohnishi T, Shima K, Semba I, Bandow K, Matsuguchi T.
タイトル Low intensity pulsed ultrasound (LIPUS) maintains osteogenic potency by the increased expression and stability of Nanog through spleen tyrosine kinase (Syk) activation.
ジャーナル Cell Signal
Abstract Mesenchymal stem cells (MSCs) are a powerful tool for cell-based, clinical therapies like bone regeneration. Therapeutic use of cell transplantation requires many cells, however, the expansion process needed to produce large quantities of cells reduces the differentiation potential of MSCs. Here, we examined the protective effects of low intensity pulsed ultrasound (LIPUS) on the maintenance of osteogenic potency. Primary osteoblastic cells were serially passaged between 2 and 12 times with daily LIPUS treatment. We found that LIPUS stimulation maintains osteogenic differentiation capacity in serially passaged cells, as characterized by improved matrix mineralization and Osteocalcin mRNA expression. Decreased expression of Nanog, Sox2, and Msx2, and increased expression of Pparg2 from serial passaging was recovered in LIPUS-stimulated cells. We found that LIPUS stimulation not only increased but also sustained expression of Nanog in primary osteoblasts and ST2 cells, a mouse mesenchymal stromal cell line. Nanog overexpression in serially passaged cells mimicked the recuperative effects of LIPUS on osteogenic potency, highlighting the important role of Nanog in LIPUS stimulation. Additionally, we found that spleen tyrosine kinase (Syk) is an important signaling molecule to induce Nanog expression in LIPUS-stimulated cells. Syk activation was regulated by both Rho-associated kinase 1 (ROCK1) and extracellular ATP in a paracrine manner. Interestingly, the LIPUS-induced increase in Nanog mRNA expression was regulated by ATP-P2X4-Syk Y323 activation, while the improvement of Nanog protein stability was controlled by the ROCK1-Syk Y525/526 pathway. Taken together, these results indicate that LIPUS stimulation recovers and maintains the osteogenic potency of serially passaged cells through a Syk-Nanog axis.
巻・号 62
ページ 109345
公開日 2019-10-1
DOI 10.1016/j.cellsig.2019.109345
PII S0898-6568(19)30141-X
PMID 31228531
MeSH Animals Cell Differentiation / radiation effects Gene Expression Regulation, Developmental / radiation effects Mesenchymal Stem Cells / metabolism* Mesenchymal Stem Cells / radiation effects Mice Nanog Homeobox Protein / genetics* Osteoblasts / radiation effects Osteogenesis / genetics* Osteogenesis / radiation effects SOXB1 Transcription Factors / genetics Syk Kinase / genetics* Ultrasonic Waves rho-Associated Kinases / genetics*
IF 3.968
引用数 2
リソース情報
ヒト・動物細胞 ST2(RCB0224)