Reference - Detail
RRC ID | 57852 |
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Author | Takeuchi T, Koinuma S, Yokosawa H, Arata Y. |
Title | An Approach for the Identification of Proteins Modified with ISG15. |
Journal | Methods Mol Biol |
Abstract |
Interferon-stimulated gene 15 (ISG15) encodes a protein that is most upregulated by type I interferon stimulation and, upon activation, is conjugated to various target proteins in a process known as ISGylation. ISGylation has been shown to have roles in various biological phenomena such as viral infection and cancer. To gain further insight into the function of ISGylation, it would be useful to be able to identify ISGylated proteins. Here, we describe a method for the identification of proteins modified with ISG15. This method involves the generation of stable ISG15-transfectant cells, followed by affinity purification, and then identification of the ISGylated proteins by mass spectrometry. |
Volume | 1934 |
Pages | 235-246 |
Published | 2019-1-1 |
DOI | 10.1007/978-1-4939-9055-9_15 |
PMID | 31256383 |
MeSH | Animals Cytokines / chemistry Cytokines / genetics Cytokines / isolation & purification Cytokines / metabolism* Humans Mice Peptides / chemistry Peptides / metabolism Protein Processing, Post-Translational RAW 264.7 Cells Recombinant Fusion Proteins Ubiquitins / chemistry Ubiquitins / genetics Ubiquitins / isolation & purification Ubiquitins / metabolism* |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) |