Reference - Detail
| RRC ID | 57853 |
|---|---|
| Author | Maehana S, Matsumoto Y, Kojima F, Kitasato H. |
| Title | Interleukin-24 Transduction Modulates Human Prostate Cancer Malignancy Mediated by Regulation of Anchorage Dependence. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND:Hormone therapy and chemotherapy are not effective for castrate-resistant prostate cancer, thus development of novel treatment strategies is required. Gene therapy involving transient high-copy transfection of interleukin (IL)-24 with an adenoviral vector can exert antitumor activity; however, the effects of stable IL-24 transfection are not fully understood. The aim of this study was to investigate the effects of IL-24 overexpression in prostate cancer cells, in vitro. MATERIALS AND METHODS:DU145 cells were transfected the IL-24 gene using a retroviral vector. Apoptosis induction was investigated by the cell death detection ELISA, and the gene expression was analyzed by real time RT-PCR. RESULTS:IL-24 transduction suppressed the growth of prostate cancer and induced tumor cell apoptosis. In addition, up-regulation of epithelial markers and down-regulation of mesenchymal markers were noted, suggesting that tumor aggressiveness was reduced. CONCLUSION:Introduction of IL-24 displays antitumor activity both by induction of apoptosis and regulation of anchorage dependence. |
| Volume | 39(7) |
| Pages | 3719-3725 |
| Published | 2019-7-1 |
| DOI | 10.21873/anticanres.13520 |
| PII | 39/7/3719 |
| PMID | 31262898 |
| MeSH | Apoptosis Cell Proliferation Humans Interleukins / genetics* Male Prostatic Neoplasms / genetics* Prostatic Neoplasms / pathology* Transduction, Genetic |
| IF | 1.994 |
| Times Cited | 0 |
| Resource | |
| Human and Animal Cells | DU145(RCB2143) |