RRC ID 57859
Author Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.
Title Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.
Journal Viruses
Abstract The 5' untranslated region (UTR) of the RNA genomes of enteroviruses possesses an internal ribosome entry site (IRES) that directs translation of the mRNA by binding to ribosomes. Infection with enterovirus D68 causes respiratory symptoms and is sometimes associated with neurological disorders. The number of reports of the viral infection and neurological disorders has increased in 2010s, although the reason behind this phenomenon remains unelucidated. In this study, we investigated the evolutionary and functional diversity of the 5' UTR of recently circulating strains of the virus. Genomic sequences of 374 viral strains were acquired and subjected to phylogenetic analysis. The IRES activity of the viruses was measured using a luciferase reporter assay. We found a highly conserved sequence in the 5' UTR and also identified the location of variable sites in the predicted RNA secondary structure. IRES activities differed among the strains in some cell lines, including neuronal and respiratory cells, and were especially high in strains of a major lineage from the recent surge. The effect of mutations in the 5' UTR should be studied further in the future for better understanding of viral pathogenesis.
Volume 11(7)
Published 2019-7-8
DOI 10.3390/v11070626
PII v11070626
PMID 31288421
PMC PMC6669567
MeSH 5' Untranslated Regions / genetics* 5' Untranslated Regions / physiology* Cell Line Enterovirus D, Human / genetics* Enterovirus D, Human / physiology Evolution, Molecular* Genetic Variation* Internal Ribosome Entry Sites / genetics* Internal Ribosome Entry Sites / physiology Nucleic Acid Conformation Phylogeny RNA, Messenger / metabolism RNA, Viral / chemistry RNA, Viral / genetics Ribosomes Viral Proteins / genetics
IF 3.811
Times Cited 0
Human and Animal Cells 293T(RCB2202) HEp-2(RCB1889) A172(RCB2530)