RRC ID 57873
著者 Crawford M, Leclerc V, Barr K, Dagnino L.
タイトル Essential Role for Integrin-Linked Kinase in Melanoblast Colonization of the Skin.
ジャーナル J Invest Dermatol
Abstract Melanocytes are pigment-producing cells found in the skin and other tissues. Alterations in the melanocyte lineage give rise to a plethora of human diseases, from neurocristopathies and pigmentation disorders to melanoma. During embryogenesis, neural crest cell subsets give rise to two waves of melanoblasts, which migrate dorsolaterally, hone to the skin, and differentiate into melanocytes. However, the mechanisms that govern colonization of the skin by the first wave of melanoblasts are poorly understood. Here we report that targeted inactivation of the integrin-linked kinase gene in first wave melanoblasts causes defects in the ability of these cells to form long pseudopods, to migrate, and to proliferate in vivo. As a result, integrin-linked kinase-deficient melanoblasts fail to populate normally the developing epidermis and hair follicles. We also show that defects in motility and dendricity occur upon integrin-linked kinase gene inactivation in mature melanocytes, causing abnormalities in cell responses to the extracellular matrix substrates collagen I and laminin 332. Significantly, the ability to form long protrusions in mutant cells in response to collagen is restored in the presence of constitutively active Rac1, suggesting that an integrin-linked kinase-Rac1 nexus is likely implicated in melanocytic cell establishment, dendricity, and functions in the skin.
巻・号 140(2)
ページ 425-434.e10
公開日 2020-2-1
DOI 10.1016/j.jid.2019.07.681
PII S0022-202X(19)32543-6
PMID 31330146
MeSH Animals Cell Differentiation / physiology* Cell Line Cell Movement / physiology Embryo, Mammalian Embryonic Stem Cells / physiology* Extracellular Matrix / metabolism Hair Follicle / cytology Hair Follicle / embryology Hair Follicle / metabolism Melanocytes / physiology* Mice Mice, Knockout Neural Crest / physiology* Neuropeptides / metabolism Primary Cell Culture Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Pseudopodia / metabolism rac1 GTP-Binding Protein / metabolism
IF 6.29
引用数 0
リソース情報
ヒト・動物細胞 ST2(RCB0224)