| RRC ID |
57876
|
| 著者 |
Morito K, Shimizu R, Kitamura N, Park SB, Kishino S, Ogawa J, Fukuta T, Kogure K, Tanaka T.
|
| タイトル |
Gut microbial metabolites of linoleic acid are metabolized by accelerated peroxisomal β-oxidation in mammalian cells.
|
| ジャーナル |
Biochim Biophys Acta Mol Cell Biol Lipids
|
| Abstract |
Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 h. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity.
|
| 巻・号 |
1864(11)
|
| ページ |
1619-1628
|
| 公開日 |
2019-11-1
|
| DOI |
10.1016/j.bbalip.2019.07.010
|
| PII |
S1388-1981(19)30130-1
|
| PMID |
31351225
|
| MeSH |
Acylation
Animals
CHO Cells
Caco-2 Cells
Cricetulus
Gastrointestinal Microbiome*
Humans
Lactobacillus plantarum / metabolism*
Linoleic Acid / metabolism*
Oxidation-Reduction
Peroxisomes / metabolism*
|
| IF |
4.402
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
MKN74(RCB1002)
CACO-2(RCB0988)
CHO-K1 |
