Reference - Detail
| RRC ID | 57892 |
|---|---|
| Author | Zhang R, Inoue Y, Konno T, Ishihara K. |
| Title | Hybridization of a phospholipid polymer hydrogel with a natural extracellular matrix using active cell immobilization. |
| Journal | Biomater Sci |
| Abstract |
Three-dimensional tissue organization is still an obstacle in the field of tissue engineering, which generally involves cell immobilization, proliferation, and organization. As an artificial extracellular matrix (ECM) for providing a suitable environment of cells to construct tissues, combination of cytocompatible polymer hydrogels and natural ECM produced by the immobilized cells was considered. In this research, we designed a spontaneously forming hydrogel system between two water-soluble polymers for the immobilization of cells. These polymers were poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-N-succinimidyloxycarbonyl tetra(ethylene glycol)methacrylate) (PMBVS) and poly(vinyl alcohol) (PVA) to form a PMBVS/PVA hydrogel in a cell culture medium under mild conditions. Basic fibroblast growth factor (bFGF) was conjugated with PMBVS (PMBV-bFGF). To enhance the growth of the immobilized cells, mouse fibroblast L929 cells were immobilized in the PMBVS/PVA hydrogel and the PMBV-bFGF/PVA hydrogel, and their proliferation and secretion of the ECM under stimulation with bFGF was observed. The ECM infiltrated and replaced the hydrogel, resulting in the formation of a hybrid hydrogel with the ECM and laden cells. |
| Volume | 7(7) |
| Pages | 2793-2802 |
| Published | 2019-6-25 |
| DOI | 10.1039/c9bm00093c |
| PMID | 31044192 |
| MeSH | Animals Biocompatible Materials / chemistry Biocompatible Materials / pharmacology* Cell Line Cell Proliferation / drug effects Cells, Immobilized / cytology* Cells, Immobilized / drug effects* Extracellular Matrix / drug effects* Extracellular Matrix / metabolism Fibroblast Growth Factor 2 / chemistry Hydrogels / chemistry* Mice Phospholipids / chemistry* Polymers / chemistry* Polyvinyl Alcohol / chemistry Rheology Solubility Water / chemistry |
| IF | 6.183 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | L929 |