| RRC ID |
57931
|
| 著者 |
May Phyu Thein Maw, Panadda Phattanawasin, Uthai Sotanaphun, Nusara Piyapolrungroj
|
| タイトル |
Natural Furanocoumarin as Potential Oral Absorption Enhancer
|
| ジャーナル |
Key Engineering Materials
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| Abstract |
Bioavailability of orally administered drugs can be influenced by many factors. Poor drug absorption across the intestinal membrane is one of the factors that contribute to low bioavailability of drugs. It has been suggested that the metabolism/active efflux in the small intestine is involved in the poor absorption of many drugs. Intestinal CYP3A4 and P-gp work coordinately to reduce the intracellular concentration of drugs. Recently, bioenhancers have been identified and extensively studied. The aim of this study was to evaluate natural furanocoumarins found in juices of common lime and kaffir lime as the potential enhancers for oral delivery by means of modulating CYP3A4 and/or P-gp activities. The role of isolated furanocoumarins on CYP3A4 was assessed by testosterone 6β-hydroxylation reaction, while the effect on P-gp was investigated using R123 and CAM uptake studies in Caco-2, as well as LLC-PK1 and LLC-GA5-Col300. In the present study, we demonstrated that isopimpinellin isolated from common lime is the best CYP3A4 inhibitor among 4 isolated furanocoumarins, implying that isopimpinellin would possibly act as a bioenhancer by inhibiting pre-systemic metabolism. 6’,7’-Dihydroxybergamottin found in kaffir lime is a dual inhibitor of CYP3A4 and P-gp, suggest that it could potentially be used as a bioenhancer by inhibiting both pre-systemic metabolism and efflux mechanism. However, in vivo study should be further conducted to confirm these effects in the body.
|
| 巻・号 |
819
|
| ページ |
63-69
|
| 公開日 |
2019-8-1
|
| DOI |
10.4028/www.scientific.net/KEM.819.63
|
| リソース情報 |
| ヒト・動物細胞 |
LLC-GA5-CoL150(RCB0871)
LLC-GA5-CoL300(RCB0872) |
