| RRC ID |
57949
|
| Author |
David BG, Fujita H, Yasuda K, Okamoto K, Panina Y, Ichinose J, Sato O, Horie M, Ichimura T, Okada Y, Watanabe TM.
|
| Title |
Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells.
|
| Journal |
Stem Cell Res
|
| Abstract |
Pluripotency of mouse embryonic stem cells is regulated by transcription factor regulatory networks as well as mechanical stimuli sensed by the cells. It has been unclear how the mechanical strain applied to the plasma membrane is transferred to the nucleus in mouse embryonic stem cells (mESCs). We here investigated the machinery of the mechanotransduction based on the finding that spontaneous differentiation of mESCs was inhibited with the downregulation of ROCK2 in cells attached to soft substrates. On examining the effects of actin bindings to both focal adhesions and cell junctions in cells on soft substrates, co-localization of actin filaments and α-catenin, which links actin to E-cadherin, decreased after differentiation induction. Also, disrupting actin-nucleus mechanical link through dominant negative assay of Nesprins helps to sustain the pluripotency genes; thus, revealing that mechanical strain relayed by actin-Nesprin connection is required for the initiation of the differentiation process.
|
| Volume |
41
|
| Pages |
101614
|
| Published |
2019-12-1
|
| DOI |
10.1016/j.scr.2019.101614
|
| PII |
S1873-5061(19)30244-2
|
| PMID |
31715427
|
| MeSH |
Actin Cytoskeleton / pathology*
Animals
Cadherins / metabolism
Cell Differentiation*
Cell Line
Cell Nucleus / metabolism*
Gene Expression Regulation, Enzymologic
Mice
Mouse Embryonic Stem Cells / cytology
Mouse Embryonic Stem Cells / metabolism*
alpha Catenin / metabolism
rho-Associated Kinases / biosynthesis
|
| IF |
4.495
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
E14tg2a(AES0135) |
