RRC ID 57988
著者 Chikae S, Kubota A, Nakamura H, Oda A, Yamanaka A, Akagi T, Akashi M.
タイトル Three-dimensional bioprinting human cardiac tissue chips of using a painting needle method.
ジャーナル Biotechnol Bioeng
Abstract Three-dimensional (3D) printers are attracting attention as a method for arranging and building cells in three dimensions. Bioprinting technology has potential in tissue engineering for the fabrication of scaffolds, cells, and tissues. However, these various printing technologies have limitations with respect to print resolution and due to the characteristics of bioink such as viscosity. We report a method for constructing of 3D tissues with a "microscopic painting device using a painting needle method" that, when used with the layer-by-layer (LbL) cell coating technique, replaces conventional methods. This method is a technique of attaching the high viscosity bioink to the painting needle tip and arranging it on a substrate, and can construct 3D tissues without damage to cells. Cell viability is the same before and after painting. We used this biofabrication device to construct 3D cardiac tissue (LbL-3D Heart) using human-induced pluripotent stem cell-derived cardiomyocytes. The constructed LbL-3D Heart chips had multiple layers with a thickness of 60 µm, a diameter of 1.1 mm, and showed synchronous beating (50-60 beats per min). The aforementioned device and method of 3D tissue construction can be applied to various kinds of tissue models and would be a useful tool for pharmaceutical applications.
巻・号 116(11)
ページ 3136-3142
公開日 2019-11-1
DOI 10.1002/bit.27126
PMID 31369146
MeSH Bioprinting* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Myocardium / cytology Myocardium / metabolism* Needles* Printing, Three-Dimensional*
IF 4.002
引用数 1
リソース情報
ヒト・動物細胞 253G1(HPS0002)