RRC ID 57990
著者 Lin PH, Chang CC, Wu KH, Shih CK, Chiang W, Chen HY, Shih YH, Wang KL, Hong YH, Shieh TM, Hsia SM.
タイトル Dietary Glycotoxins, Advanced Glycation End Products, Inhibit Cell Proliferation and Progesterone Secretion in Ovarian Granulosa Cells and Mimic PCOS-like Symptoms.
ジャーナル Biomolecules
Abstract Women with polycystic ovary syndrome (PCOS) have been reported to have an elevated serum advanced glycation end product (AGE) level. However, the effect of AGEs on the pathophysiological ovarian granulosa cells of PCOS is still unclear. In this study, five indented BSA-derived AGE products were used to evaluate their effect on the function of human granulosa cells. We found that the proliferation of both primary human ovarian granulosa (hGC) cells and human granulosa-like tumor (KGN) cells were inhibited by treatment with these five AGE products. The progesterone secretion level was also reduced in both hGC and KGN cells by treatment with these AGE products through downregulation of LH receptor/cAMP regulatory activity. The granulosa cell layer and serum progesterone level were reduced in rats by treatment with MG-BSA; moreover, an increased number of follicle cysts and an irregular estrous cycle were observed. MG-BSA treatment had a similar effect on the phenotypes of the DHEA-induced PCOS model. Additionally, the insulin resistance and hepatic lesions seen in the DHEA-induced PCOS model were observed in the MG-BSA treatment group. Taken together, we found that AGEs exert a toxic effect on ovarian granulosa cells, ovarian morphology, and the estrous cycle that mimics the DHEA-induced PCOS phenotypes.
巻・号 9(8)
公開日 2019-7-31
DOI 10.3390/biom9080327
PII biom9080327
PMID 31370285
PMC PMC6723748
MeSH Animals Cell Proliferation / drug effects Dehydroepiandrosterone / pharmacology Diet* Female Glucose / metabolism Glycation End Products, Advanced / pharmacology* Granulosa Cells / drug effects* Granulosa Cells / metabolism Granulosa Cells / pathology Homeostasis / drug effects Polycystic Ovary Syndrome / metabolism* Polycystic Ovary Syndrome / pathology* Progesterone / metabolism* Rats Rats, Sprague-Dawley
IF 4.694
引用数 5
リソース情報
ヒト・動物細胞 KGN(RCB1154)