論文 - 詳細
| RRC ID | 57997 |
|---|---|
| 著者 | Ogawa Y, Takahashi N, Takemoto T, Nishiume T, Suzuki M, Ishiguro N, Kojima T. |
| タイトル | Hyaluronan promotes TRPV4-induced chondrogenesis in ATDC5 cells. |
| ジャーナル | PLoS One |
| Abstract |
Hyaluronan (HA) is an extracellular matrix glycosaminoglycan essential for the homeostasis of cartilage-related tissues. Intracellular adhesion molecule-1 (ICAM-1) and CD44 have been identified as receptors for HA. Recently, transient receptor potential vanilloid 4 (TRPV4) has emerged as a potential research target in several areas of physiology. TRPV4 is a Ca2+-permeable, non-selective cation channel that appears to have mechanosensory or osmosensory roles in several musculoskeletal tissues. HA and TRPV4 play key roles in chondrogenesis; however, it has remained unclear whether they have interactive effects on chondrogenesis and, if so, how do they interact with each other? This study investigated the relationship between HA, its receptors ICAM-1 and CD44, and TRPV4 in the chondrogenic pathway using the ATDC5 cell line. It was found that the presence of HA is required for TRPV4-induced chondrogenesis. Loss of HA suppressed TRPV4-induced expression of the chondrogenic markers, SOX9 and Aggrecan. Moreover, HA affects TRPV4-induced chondrogenic development via each of ICAM-1 and CD44 partially. In conclusion, for the first time, the existence of an interaction between HA, its receptor ICAM-1 and CD44, and TRPV4-activity in chondrogenesis in the ATDC5 cell line was reported. TRPV4 is known to function as a mechanosensory channel in several musculoskeletal tissues. Therefore, findings of this study may suggest the existence of a molecular mechanism that underlies the interactive effects of HA and mechanical loading on joint chondrogenesis. |
| 巻・号 | 14(8) |
| ページ | e0219492 |
| 公開日 | 2019-1-1 |
| DOI | 10.1371/journal.pone.0219492 |
| PII | PONE-D-18-28391 |
| PMID | 31393869 |
| PMC | PMC6687147 |
| MeSH | Animals Cartilage / metabolism Cell Differentiation / drug effects Cell Line Chondrocytes / metabolism Chondrogenesis / physiology* Glycosaminoglycans / metabolism Hyaluronan Receptors / metabolism Hyaluronic Acid / metabolism* Hyaluronic Acid / physiology Intercellular Adhesion Molecule-1 / metabolism Mice TRPV Cation Channels / metabolism* TRPV Cation Channels / physiology |
| IF | 2.74 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |