Reference - Detail
| RRC ID | 57999 |
|---|---|
| Author | Kawai Y, Kaneko S. |
| Title | Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC. |
| Journal | Methods Mol Biol |
| Abstract |
In this chapter, we describe redifferentiation procedures from iPSCs to CD8αβ+ cytotoxic T cells in 10 T1/2 and OP9/DL1 feeder condition. iPSC used here is derived from T-cell clone (T-iPSC), which has lost naïve phenotype and acquired exhaustion/senescence phenotype during cloning process (Note 1). On the other hand, redifferentiated T cells (T-iPSC-Ts) reacquire naïve phenotype (CD45RA+CD45RO-CCR7+CD62L+), which are reportedly critical for in vivo persistence of infused T cells and greatly affect therapeutic efficacy of adoptive immunotherapy. Indeed, T-iPSC-Ts exhibit much superior proliferative capacity while retaining equivalent effector function compared to parental T-cell clones. Here, we demonstrate the methodology to produce naïve-like T-iPSC-Ts, which could be potent cell source for adoptive immunotherapy. |
| Volume | 2048 |
| Pages | 71-75 |
| Published | 2019-1-1 |
| DOI | 10.1007/978-1-4939-9728-2_7 |
| PMID | 31396930 |
| MeSH | Animals Cell Culture Techniques / instrumentation Cell Culture Techniques / methods* Cell Differentiation Cell Line Coculture Techniques / instrumentation Coculture Techniques / methods Humans Immunologic Memory Immunotherapy, Adoptive / methods Induced Pluripotent Stem Cells Mesenchymal Stem Cells Mice T-Lymphocytes, Cytotoxic / physiology* T-Lymphocytes, Cytotoxic / transplantation |
| Times Cited | 0 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) |