RRC ID 58026
Author Hasan I, Asaduzzaman AKM, Swarna RR, Fujii Y, Ozeki Y, Uddin MB, Kabir SR.
Title MytiLec-1 Shows Glycan-Dependent Toxicity against Brine Shrimp Artemia and Induces Apoptotic Death of Ehrlich Ascites Carcinoma Cells In Vivo.
Journal Mar Drugs
Abstract MytiLec-1, a 17 kDa lectin with β-trefoil folding that was isolated from the Mediterranean mussel (Mytilus galloprovincialis) bound to the disaccharide melibiose, Galα(1,6) Glc, and the trisaccharide globotriose, Galα(1,4) Galβ(1,4) Glc. Toxicity of the lectin was found to be low with an LC50 value of 384.53 μg/mL, determined using the Artemia nauplii lethality assay. A fluorescence assay was carried out to evaluate the glycan-dependent binding of MytiLec-1 to Artemia nauplii. The lectin strongly agglutinated Ehrlich ascites carcinoma (EAC) cells cultured in vivo in Swiss albino mice. When injected intraperitoneally to the mice at doses of 1.0 mg/kg/day and 2.0 mg/kg/day for five consecutive days, MytiLec-1 inhibited 27.62% and 48.57% of cancer cell growth, respectively. Antiproliferative activity of the lectin against U937 and HeLa cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro in RPMI-1640 medium. MytiLec-1 internalized into U937 cells and 50 μg/mL of the lectin inhibited their growth of to 62.70% whereas 53.59% cell growth inhibition was observed against EAC cells when incubated for 24 h. Cell morphological study and expression of apoptosis-related genes (p53, Bax, Bcl-X, and NF-κB) showed that the lectin possibly triggered apoptosis in these cells.
Volume 17(9)
Published 2019-8-28
DOI 10.3390/md17090502
PII md17090502
PMID 31466257
PMC PMC6780975
MeSH Animals Apoptosis / drug effects Artemia / drug effects Biological Products / chemistry Biological Products / pharmacology* Biological Products / therapeutic use Carcinoma, Ehrlich Tumor / drug therapy* Carcinoma, Ehrlich Tumor / pathology Cell Proliferation / drug effects Disaccharides / chemistry Disaccharides / pharmacology* Disaccharides / therapeutic use Drug Screening Assays, Antitumor HeLa Cells Humans Injections, Intraperitoneal Lectins / chemistry Lectins / pharmacology* Lectins / therapeutic use Melibiose / chemistry Mice Mytilus / chemistry* Toxicity Tests Trisaccharides / chemistry Trisaccharides / pharmacology* Trisaccharides / therapeutic use U937 Cells
IF 4.073
Times Cited 1
Human and Animal Cells U937 HeLa