論文 - 詳細
| RRC ID | 58030 |
|---|---|
| 著者 | Kawamura E, Hibino M, Harashima H, Yamada Y. |
| タイトル | Targeted mitochondrial delivery of antisense RNA-containing nanoparticles by a MITO-Porter for safe and efficient mitochondrial gene silencing. |
| ジャーナル | Mitochondrion |
| Abstract |
Mitochondrial gene therapy will be needed to treat mitochondrial diseases. We previously demonstrated mitochondrial gene silencing by the mitochondrial delivery of antisense RNA oligonucleotide (ASO) targeting mtDNA-encoded mRNA using a MITO-Porter, a liposomal nano carrier system designed for mitochondrial delivery. Here, we report on the efficient packaging of ASO in the MITO-Porter via a nanoparticle packaging method, which showed a 10-fold higher packaging efficiency than the conventional method. The constructed carrier showed a decrease in the target mRNA levels and ATP production. These results indicate that such a MITO-Porter has potential for use in therapies designed to regulate mitochondrial function. |
| 巻・号 | 49 |
| ページ | 178-188 |
| 公開日 | 2019-11-1 |
| DOI | 10.1016/j.mito.2019.08.004 |
| PII | S1567-7249(18)30286-1 |
| PMID | 31472283 |
| MeSH | Gene Silencing* Gene Transfer Techniques* Genes, Mitochondrial* HeLa Cells Humans Mitochondria* / genetics Mitochondria* / metabolism Nanoparticles / chemistry* RNA, Antisense* / chemistry RNA, Antisense* / genetics RNA, Antisense* / pharmacology |
| IF | 3.992 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |