RRC ID 58046
著者 Horie M, Tabei Y, Sugino S, Eguchi K, Chiba R, Tajika M.
タイトル Comparison of proinflammatory potential of needle-shaped materials: aragonite and potassium titanate whisker.
ジャーナル Arch Toxicol
Abstract Among the crystal forms of calcium carbonate, aragonite has needle-like shape. Although materials with needle-shaped crystals are associated with pulmonary toxicity, the toxic activity of aragonite is unclear. Therefore, proinflammatory potential of aragonite, neutralized aragonite and potassium titanate whisker was evaluated. The cellular effects of aragonite were weaker than those of potassium titanate whisker. Aragonite treatment induced the expression of chemokines in A549 cells and macrophages. Although aragonite exhibited proinflammatory effects in vitro, pulmonary inflammation was not observed in vivo after intratracheal administration of aragonite in mice. We did not observe the induction of inflammatory cytokine secretion or tissue lesion in the lungs of mice after administration of aragonite. Potassium titanate whisker treatment induced chemokine secretion in vitro. An increase in the number of neutrophils was observed in the mice lung tissue after administration of potassium titanate whisker. Aragonite and neutralized aragonite both induced an increase in the levels of intracellular calcium, but the levels were significantly higher in cells treated with aragonite than in cells treated with neutralized aragonite. These results suggested that intracellular calcium release mediates the cellular effects of aragonite. The toxicity of aragonite based on its needle-like structure was also not observed.
巻・号 93(10)
ページ 2797-2810
公開日 2019-10-1
DOI 10.1007/s00204-019-02556-w
PII 10.1007/s00204-019-02556-w
PMID 31493027
MeSH A549 Cells Animals Calcium / metabolism Calcium Carbonate / chemistry Calcium Carbonate / toxicity* Chemokines / metabolism Humans Inflammation / chemically induced* Inflammation / pathology Macrophages / drug effects* Macrophages / pathology Male Mice Mice, Inbred C57BL Neutrophils / metabolism Titanium / chemistry Titanium / toxicity*
IF 5.059
引用数 0
リソース情報
ヒト・動物細胞 A549