| RRC ID |
58050
|
| 著者 |
Zhou X, Bai H, Kataoka M, Ito M, Muramatsu M, Suzuki T, Li TC.
|
| タイトル |
Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan.
|
| ジャーナル |
Sci Rep
|
| Abstract |
New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient's vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1-70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050).
|
| 巻・号 |
9(1)
|
| ページ |
13085
|
| 公開日 |
2019-9-11
|
| DOI |
10.1038/s41598-019-49541-y
|
| PII |
10.1038/s41598-019-49541-y
|
| PMID |
31511622
|
| PMC |
PMC6739320
|
| MeSH |
Adolescent
Adult
Aged
Animals
Antibodies, Viral / blood
Antibodies, Viral / immunology
Capsid Proteins / chemistry*
Capsid Proteins / immunology
Child
Child, Preschool
Cross Reactions
Female
Humans
Immune Sera / immunology
Infant
Japan
Male
Middle Aged
Polyomavirus / immunology
Polyomavirus / physiology*
Protein Aggregates*
Seroepidemiologic Studies
Sf9 Cells
Spodoptera
Young Adult
|
| IF |
3.998
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
Sf9(RCB0563) |
