論文 - 詳細
| RRC ID | 58050 |
|---|---|
| 著者 | Zhou X, Bai H, Kataoka M, Ito M, Muramatsu M, Suzuki T, Li TC. |
| タイトル | Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan. |
| ジャーナル | Sci Rep |
| Abstract |
New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient's vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1-70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050). |
| 巻・号 | 9(1) |
| ページ | 13085 |
| 公開日 | 2019-9-11 |
| DOI | 10.1038/s41598-019-49541-y |
| PII | 10.1038/s41598-019-49541-y |
| PMID | 31511622 |
| PMC | PMC6739320 |
| MeSH | Adolescent Adult Aged Animals Antibodies, Viral / blood Antibodies, Viral / immunology Capsid Proteins / chemistry* Capsid Proteins / immunology Child Child, Preschool Cross Reactions Female Humans Immune Sera / immunology Infant Japan Male Middle Aged Polyomavirus / immunology Polyomavirus / physiology* Protein Aggregates* Seroepidemiologic Studies Sf9 Cells Spodoptera Young Adult |
| IF | 3.998 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |