| RRC ID |
58053
|
| Author |
Lee DS, Luo C, Zhou J, Chandran S, Rivkin A, Bartlett A, Nery JR, Fitzpatrick C, O'Connor C, Dixon JR, Ecker JR.
|
| Title |
Simultaneous profiling of 3D genome structure and DNA methylation in single human cells.
|
| Journal |
Nat Methods
|
| Abstract |
Dynamic three-dimensional chromatin conformation is a critical mechanism for gene regulation during development and disease. Despite this, profiling of three-dimensional genome structure from complex tissues with cell-type specific resolution remains challenging. Recent efforts have demonstrated that cell-type specific epigenomic features can be resolved in complex tissues using single-cell assays. However, it remains unclear whether single-cell chromatin conformation capture (3C) or Hi-C profiles can effectively identify cell types and reconstruct cell-type specific chromatin conformation maps. To address these challenges, we have developed single-nucleus methyl-3C sequencing to capture chromatin organization and DNA methylation information and robustly separate heterogeneous cell types. Applying this method to >4,200 single human brain prefrontal cortex cells, we reconstruct cell-type specific chromatin conformation maps from 14 cortical cell types. These datasets reveal the genome-wide association between cell-type specific chromatin conformation and differential DNA methylation, suggesting pervasive interactions between epigenetic processes regulating gene expression.
|
| Volume |
16(10)
|
| Pages |
999-1006
|
| Published |
2019-10-1
|
| DOI |
10.1038/s41592-019-0547-z
|
| PII |
10.1038/s41592-019-0547-z
|
| PMID |
31501549
|
| PMC |
PMC6765423
|
| MeSH |
Algorithms
Chromatin / metabolism
DNA Methylation*
Datasets as Topic
Epigenesis, Genetic
Gene Expression Regulation
Genome, Human*
Genome-Wide Association Study
Humans
Single-Cell Analysis*
|
| IF |
30.822
|
| Times Cited |
7
|
| Resource |
| Human and Animal Cells |
NMuMG/Fucci2(RCB2868) |
