論文 - 詳細
| RRC ID | 58074 |
|---|---|
| 著者 | Dukovski BJ, Bračko A, Šare M, Pepić I, Lovrić J. |
| タイトル | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery. |
| ジャーナル | Acta Pharm |
| Abstract |
Oil-in-water nanoemulsions (NEs) represent one of the formulation approaches to improve eye-related bio-availability of lipophilic drugs. The potential of cationic NEs is pronounced due to the electrostatic interaction of positively charged droplets with negatively charged mucins present in the tear film, providing prolonged formulation residence at the ocular surface. The aim of this study was to develop a cationic ophthalmic NE with cationic lipid stearylamine (SA) as a carrier of a positive charge. The addition of a nonionic surfactant provided the dual electro-steric stabilization of NEs and enabled tuning of SA concentration to achieve an optimal balance between its interaction with mucins and biocompatibility. Physicochemical characterization, stability profile, in vitro mucoadhesion study and biocompatibility study employing 3D HCE-T cell-based model of corneal epithelium pointed out the NE with 0.05 % (m/m) SA as the leading formulation. Minimizing SA content while retaining droplet/mucin interactions is of great importance for efficacy and safety of future ophthalmic drug products. |
| 巻・号 | 69(4) |
| ページ | 621-634 |
| 公開日 | 2019-12-1 |
| DOI | 10.2478/acph-2019-0054 |
| PII | /j/acph.2019.69.issue-4/acph-2019-0054/acph-2019-0054.xml |
| PMID | 31639085 |
| MeSH | Administration, Ophthalmic Amines / administration & dosage Amines / chemistry* Biocompatible Materials / administration & dosage Biocompatible Materials / chemistry Biological Availability Cations / administration & dosage Cations / chemistry* Cell Line Cornea / drug effects Drug Delivery Systems / methods Emulsions / administration & dosage Emulsions / chemistry* Epithelium / drug effects Humans Lipids / administration & dosage Lipids / chemistry Nanoparticles / administration & dosage Nanoparticles / chemistry* |
| IF | 1.405 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |