| RRC ID |
58103
|
| 著者 |
Matsui S, Kataoka H, Tanaka JI, Kikuchi M, Fukamachi H, Morisaki H, Matsushima H, Mishima K, Hironaka S, Takaki T, Okahashi N, Maruoka Y, Kuwata H.
|
| タイトル |
Dysregulation of Intestinal Microbiota Elicited by Food Allergy Induces IgA-Mediated Oral Dysbiosis.
|
| ジャーナル |
Infect Immun
|
| Abstract |
Food allergy is a life-threatening response to specific foods, and microbiota imbalance (dysbiosis) in gut is considered a cause of this disease. Meanwhile, the host immune response also plays an important role in the disease. Notably, interleukin 33 (IL-33) released from damaged or necrotic intestinal epithelial cells facilitates IL-2-producing CD4 helper T (Th2) responses. However, causal relationships between the gut and oral dysbiosis and food allergy remain unknown. In this study, we analyzed effects of gut and oral dysbiosis on development of food allergy. A murine model of food allergy was established via ovalbumin (OVA) injection in BALB/c mice. Viable fecal bacteria were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). il33 expression in colon-26 mouse colon cells stimulated by isolated fecal bacteria was quantified by real-time PCR. Intestinal T cells from the mice were analyzed by flow cytometry. Salivary IgA levels were quantified by enzyme-linked immunosorbent assay (ELISA), and IgA-bound oral bacteria were detected by flow cytometry. Among fecal bacteria, the abundance of Citrobacter sp. increased in the feces of allergic mice and induced il33 expression in colon-26 cells. Orally administered Citrobacter koseri JCM1658 exacerbated systemic allergic symptoms and reduced intestinal Th17 cells. Salivary IgA and IgA-bound oral bacteria increased in the allergic mice. Based on the results described above, food allergy induced both gut and oral dysbiosis. Citrobacter sp. aggravated allergy symptoms by inducing IL-33 release from intestinal epithelial cells.
|
| 巻・号 |
88(1)
|
| 公開日 |
2019-12-17
|
| DOI |
10.1128/IAI.00741-19
|
| PII |
IAI.00741-19
|
| PMID |
31611274
|
| PMC |
PMC6921656
|
| MeSH |
Animals
Cytokines / metabolism
Disease Models, Animal
Dysbiosis*
Epithelial Cells / metabolism
Food Hypersensitivity / complications*
Gastrointestinal Tract / microbiology*
Immunoglobulin A / metabolism*
Immunologic Factors / metabolism*
Mice, Inbred BALB C
Microbiota / drug effects*
Mouth / microbiology*
|
| IF |
3.201
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
Colon-26(RCB2657) |
