論文 - 詳細
| RRC ID | 58130 |
|---|---|
| 著者 | Terao Y, Kurashina Y, Tohyama S, Fukuma Y, Fukuda K, Fujita J, Takemura K. |
| タイトル | An effective detachment system for human induced pluripotent stem cells cultured on multilayered cultivation substrates using resonance vibrations. |
| ジャーナル | Sci Rep |
| Abstract |
Clinical application of human induced pluripotent stem cells (hiPSCs) has been hampered by the lack of a practical, scalable culture system. Stacked culture plates (SCPs) have recently attracted attention. However, final cell yields depend on the efficiency of cell detachment, and inefficient cell recovery from SCPs presents a major challenge to their use. We have developed an effective detachment method using resonance vibrations (RVs) of substrates with sweeping driving frequency. By exciting RVs that have 1-3 antinodes with ultra-low-density enzyme spread on each substrate of SCPs, 87.8% of hiPSCs were successfully detached from a 5-layer SCP compared to 30.8% detached by the conventional enzymatic method. hiPSC viability was similar after either method. Moreover, hiPSCs detached by the RV method maintained their undifferentiated state. Additionally, hiPSCs after long-term culture (10 passages) kept excellent detachment efficiency, had the normal karyotypes, and maintained the undifferentiated state and pluripotency. These results indicated that the RV method has definite advantages over the conventional enzymatic method in the scalable culture of hiPSCs using SCPs. |
| 巻・号 | 9(1) |
| ページ | 15655 |
| 公開日 | 2019-10-30 |
| DOI | 10.1038/s41598-019-51944-w |
| PII | 10.1038/s41598-019-51944-w |
| PMID | 31666563 |
| PMC | PMC6821886 |
| MeSH | Animals Cell Adhesion* Cell Culture Techniques / instrumentation* Humans Induced Pluripotent Stem Cells / cytology* Mice Myoblasts / cytology Vibration* |
| IF | 3.998 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |