RRC ID 58151
Author Li K, Li M, Li W, Yu H, Sun X, Zhang Q, Li Y, Li X, Li Y, Abel ED, Wu Q, Chen H.
Title Airway epithelial regeneration requires autophagy and glucose metabolism.
Journal Cell Death Dis
Abstract Efficient repair of injured epithelium by airway progenitor cells could prevent acute inflammation from progressing into chronic phase in lung. Here, we used small molecules, genetic loss-of-function, organoid cultures, and in vivo lung-injury models to show that autophagy is essential for maintaining the pool of airway stem-like vClub cells by promoting their proliferation during ovalbumin-induced acute inflammation. Mechanistically, impaired autophagy disrupted glucose uptake in vClub progenitor cells, and either reduced accessibility to glucose or partial inhibition of glycolysis promoted the proliferative capacity of vClub progenitor cells and their daughter Club cells. However, glucose deprivation or glycolysis blockade abrogated the proliferative capacity of airway vClub cells and Club cells but promoted ciliated and goblet cell differentiation. Deficiency of glucose transporter-1 suppressed the proliferative capacity of airway progenitor cells after ovalbumin challenge. These findings suggested that autophagy and glucose metabolism are essential for the maintenance of airway epithelium at steady state and during allergic inflammation.
Volume 10(12)
Pages 875
Published 2019-11-20
DOI 10.1038/s41419-019-2111-2
PII 10.1038/s41419-019-2111-2
PMID 31748541
PMC PMC6868131
MeSH Animals Autophagy Cell Differentiation / physiology Epithelial Cells / cytology Epithelial Cells / metabolism Epithelial Cells / physiology Glucose / metabolism* Humans Lung / cytology Lung / metabolism Lung / physiology* Mice Mice, Inbred C57BL Regeneration / physiology* Stem Cells / cytology Stem Cells / metabolism Stem Cells / physiology*
IF 6.304
Times Cited 1
Mice RBRC02975