RRC ID 582
Author Gable K, Garton S, Napier JA, Dunn TM.
Title Functional characterization of the Arabidopsis thaliana orthologue of Tsc13p, the enoyl reductase of the yeast microsomal fatty acid elongating system.
Journal J Exp Bot
Abstract The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the temperature-sensitive phenotype of a yeast tsc13 mutant that is deficient in enoyl reductase activity. Furthermore, AtTSC13 is shown to interact physically with the Elo2p and Elo3p components of the yeast elongase complex. At3g55360 apparently encodes the sole enoyl reductase activity associated with microsomal fatty acid elongation in Arabidopsis. Consistent with this conclusion, AtTSC13 is ubiquitously expressed in Arabidopsis.
Volume 55(396)
Pages 543-5
Published 2004-2-1
DOI 10.1093/jxb/erh061
PII erh061
PMID 14673020
MeSH Arabidopsis / enzymology* Arabidopsis / genetics* Base Sequence DNA Primers Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) Fatty Acid Synthases / genetics Fatty Acids / metabolism* Microsomes / enzymology* Oxidoreductases / genetics* Oxidoreductases Acting on CH-CH Group Donors Polymerase Chain Reaction Saccharomyces cerevisiae Proteins / genetics*
IF 5.908
Times Cited 35
Arabidopsis / Cultured plant cells, genes pda