RRC ID 5822
Author Fukamachi K, Tanaka H, Hagiwara Y, Ohara H, Joh T, Iigo M, Alexander DB, Xu J, Long N, Takigahira M, Yanagihara K, Hino O, Saito I, Tsuda H.
Title An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas.
Journal Biochem Biophys Res Commun
Abstract Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease, which is usually diagnosed in an advanced stage. Animal PDA models which reflect the human condition are clearly necessary to develop early diagnostic tools and explore new therapeutic approaches. We have established transgenic rats carrying a mutated H- or K-ras gene (Hras250 and Kras327) controlled by Cre/loxP activation. These animals develop PDA which are histopathologically similar to that in humans. We utilized this model to identify biomarkers to detect early PDA. We report here that serum levels of Erc/Mesothelin are significantly higher in rats bearing PDA than in controls. Importantly, the levels are significantly elevated in rats before grossly visible carcinomas develop. Even in rats with very small microscopic ductal carcinoma lesions, elevated serum Erc/Mesothelin can be detected. We believe this is the first report of a pancreas tumor animal model in which pre-symptomatic lesions can be diagnosed.
Volume 390(3)
Pages 636-41
Published 2009-12-18
DOI 10.1016/j.bbrc.2009.10.019
PII S0006-291X(09)01983-4
PMID 19818733
MeSH Animals Biomarkers, Tumor / blood* Carcinoma, Pancreatic Ductal / blood Carcinoma, Pancreatic Ductal / diagnosis* Carcinoma, Pancreatic Ductal / pathology Disease Models, Animal Female GPI-Linked Proteins Genes, ras / genetics Humans Membrane Glycoproteins / blood* Mesothelin Pancreatic Neoplasms / blood Pancreatic Neoplasms / diagnosis* Pancreatic Neoplasms / pathology Rats Rats, Sprague-Dawley Rats, Transgenic
IF 2.985
Times Cited 16
DNA material pCALNL5 (RDB01862)