Reference - Detail
| RRC ID | 58228 |
|---|---|
| Author | Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H. |
| Title | Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. |
| Journal | J Neurochem |
| Abstract |
Expression of nitric oxide synthase (NOS) was studied in nine human neuroblastoma and two human glioblastoma cell lines. Neuronal NOS (n-NOS) mRNA of approximately 10 kb was detected in four of the nine neuroblastoma cell lines by northern blot analysis using human n-NOS cDNA as a probe. Expression of the n-NOS mRNA was also detected in another neuroblastoma cell line in a subsequent reverse transcriptase polymerase chain reaction (RT-PCR) study, but no n-NOS mRNA expression was observed in the other four neuroblastoma cell lines or in the glioblastoma cell lines. The level of NOS activity correlated well with that of n-NOS mRNA expression in neuroblastoma cell lines expressing n-NOS mRNA. Western blot analysis showed that the n-NOS expressed in neuroblastoma cells was a 160-kDa protein reacted with anti-n-NOS antibody. By using the RT-PCR method, a short n-NOS (n-NOS-2) mRNA with a 315-bp inframe deletion from the entire n-NOS (n-NOS-1) mRNA was detected in the human neuroblastoma cells. The structural diversity of human n-NOS mRNA was demonstrated for the first time. |
| Volume | 63(1) |
| Pages | 140-5 |
| Published | 1994-7-1 |
| DOI | 10.1046/j.1471-4159.1994.63010140.x |
| PMID | 7515942 |
| MeSH | Amino Acid Oxidoreductases / analysis Amino Acid Oxidoreductases / genetics* Amino Acid Oxidoreductases / physiology Amino Acid Sequence Base Sequence Blotting, Southern Blotting, Western DNA, Neoplasm / analysis DNA, Neoplasm / genetics Glioblastoma / chemistry Glioblastoma / enzymology Glioblastoma / pathology Humans Molecular Sequence Data Neuroblastoma / chemistry* Neuroblastoma / enzymology Neuroblastoma / pathology* Nitric Oxide Synthase Polymerase Chain Reaction RNA, Messenger / analysis* RNA, Messenger / genetics Tumor Cells, Cultured |
| IF | 4.066 |
| Times Cited | 60 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Human Neuroral NOS cDNA (RDB01387) |