| RRC ID |
58228
|
| Author |
Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H.
|
| Title |
Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines.
|
| Journal |
J Neurochem
|
| Abstract |
Expression of nitric oxide synthase (NOS) was studied in nine human neuroblastoma and two human glioblastoma cell lines. Neuronal NOS (n-NOS) mRNA of approximately 10 kb was detected in four of the nine neuroblastoma cell lines by northern blot analysis using human n-NOS cDNA as a probe. Expression of the n-NOS mRNA was also detected in another neuroblastoma cell line in a subsequent reverse transcriptase polymerase chain reaction (RT-PCR) study, but no n-NOS mRNA expression was observed in the other four neuroblastoma cell lines or in the glioblastoma cell lines. The level of NOS activity correlated well with that of n-NOS mRNA expression in neuroblastoma cell lines expressing n-NOS mRNA. Western blot analysis showed that the n-NOS expressed in neuroblastoma cells was a 160-kDa protein reacted with anti-n-NOS antibody. By using the RT-PCR method, a short n-NOS (n-NOS-2) mRNA with a 315-bp inframe deletion from the entire n-NOS (n-NOS-1) mRNA was detected in the human neuroblastoma cells. The structural diversity of human n-NOS mRNA was demonstrated for the first time.
|
| Volume |
63(1)
|
| Pages |
140-5
|
| Published |
1994-7-1
|
| DOI |
10.1046/j.1471-4159.1994.63010140.x
|
| PMID |
7515942
|
| MeSH |
Amino Acid Oxidoreductases / analysis
Amino Acid Oxidoreductases / genetics*
Amino Acid Oxidoreductases / physiology
Amino Acid Sequence
Base Sequence
Blotting, Southern
Blotting, Western
DNA, Neoplasm / analysis
DNA, Neoplasm / genetics
Glioblastoma / chemistry
Glioblastoma / enzymology
Glioblastoma / pathology
Humans
Molecular Sequence Data
Neuroblastoma / chemistry*
Neuroblastoma / enzymology
Neuroblastoma / pathology*
Nitric Oxide Synthase
Polymerase Chain Reaction
RNA, Messenger / analysis*
RNA, Messenger / genetics
Tumor Cells, Cultured
|
| IF |
4.066
|
| Times Cited |
60
|
| Resource |
| DNA material |
Human Neuroral NOS cDNA (RDB01387) |
