RRC ID 5827
Author Koto A, Kuranaga E, Miura M.
Title Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development.
Journal J. Cell Biol.
Abstract The caspases comprise a family of cysteine proteases that function in various cellular processes, including apoptosis. However, how the balance is struck between the caspases' role in cell death and their nonapoptotic functions is unclear. To address this issue, we monitored the protein turnover of an endogenous caspase inhibitor, Drosophila IAP1 (DIAP1). DIAP1 is an E3 ubiquitin ligase that promotes the ubiquitination of caspases and thereby prevents caspase activation. For this study, we developed a fluorescent probe to monitor DIAP1 turnover in the external sensory organ precursor (SOP) lineage of living Drosophila. The SOP divides asymmetrically to make the shaft, socket, and sheath cells, and the neuron that comprise each sensory organ. We found that the quantity of DIAP1 changed dramatically depending on the cell type and maturity, and that the temporal regulation of DIAP1 turnover determines whether caspases function nonapoptotically in cellular morphogenesis or cause cell death.
Volume 187(2)
Pages 219-31
Published 2009-10-19
DOI 10.1083/jcb.200905110
PII jcb.200905110
PMID 19822670
PMC PMC2768825
MeSH Animals Apoptosis Caspases / metabolism* Cell Line Cell Lineage Cell Shape Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental* Inhibitor of Apoptosis Proteins / genetics Inhibitor of Apoptosis Proteins / metabolism* Sense Organs / cytology Sense Organs / growth & development Sense Organs / metabolism Signal Transduction
IF 8.784
Times Cited 34