Reference - Detail
| RRC ID | 58287 |
|---|---|
| Author | Zhao P, You Q, Lei M. |
| Title | A CRISPR/Cas9 deletion into the phosphate transporter SlPHO1;1 reveals its role in phosphate nutrition of tomato seedlings. |
| Journal | Physiol Plant |
| Abstract |
In vascular (Arabidopsis thaliana) and non-vascular (Physcomitrella patens) plants, PHOSPHATE 1 (PHO1) homologs play important roles in the acquisition and transfer of phosphate. The tomato genome contains six genes (SlPHO1;1-SlPHO1;6) homologous to AtPHO1. The six proteins have typical characteristics of the plant PHO1 family, such as the three Syg1/Pho81/XPRI (SPX) subdomains in the N-terminal portion and one ERD1/XPR1/SYG1 (EXS) domain in the C-terminal portion. Phylogenetic analysis revealed that the SlPHO1 family is subdivided into three clusters. A pairwise comparison indicated that SlPHO1;1 showed the highest level of sequence identity/similarity (67.39/76.21%) to AtPHO1. SlPHO1;1 deletion mutants induced by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 displayed typical phenotypes of Pi starvation, such as decreased shoot fresh weight and increased root fresh weight, therefore having a greater root-to-shoot ratio. Mutants also accumulated more anthocyanin and had more soluble Pi content in the root and less in the shoot. These results indicate that SlPHO1;1 plays an important role in Pi transport in the tomato at seedling stage. |
| Volume | 167(4) |
| Pages | 556-563 |
| Published | 2019-12-1 |
| DOI | 10.1111/ppl.12897 |
| PMID | 30537089 |
| MeSH | CRISPR-Cas Systems* Gene Expression Regulation, Plant Phosphate Transport Proteins / genetics* Phosphates / physiology* Phylogeny Plant Proteins / genetics* Plants, Genetically Modified Seedlings / genetics Seedlings / physiology Solanum lycopersicum / genetics* Solanum lycopersicum / physiology |
| IF | 4.148 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Tomato | TOMJPF00001 (Micro-Tom) |