RRC ID 58303
著者 Tsukiji N, Amano T, Shiroishi T.
タイトル A novel regulatory element for Shh expression in the lung and gut of mouse embryos.
ジャーナル Mech Dev
Abstract Hedgehog (Hh) signaling plays pivotal roles in morphogenesis of several embryonic tissues, including the primitive gut. In the mouse embryo, Sonic hedgehog (Shh) is expressed in endodermal epithelia from the oral cavity to the intestine, and contributes to cell proliferation in the underlying mesenchyme and subsequent differentiation into the gastrointestinal smooth muscle. Three evolutionary conserved non-coding sequences in the region upstream of the Shh coding sequence contain endoderm-specific enhancers for Shh expression. Although Shh expression in the endodermal epithelial lining is mostly attributed to these three enhancers, none of them regulates gene expression in the gastroesophageal epithelium. Here, we found that a 1.7Kb fragment located 100Kb upstream of the Shh coding sequence contains a functional element for Shh expression in endodermal organs, including the esophagus and stomach. Compared with the three known endodermal enhancers, this novel enhancer shows less evolutionary conservation, even among rodents. In mouse embryonic endodermal tissues, the seamless expression of Shh is achieved by a patchwork of multiple enhancers with different rates of evolution.
巻・号 131
ページ 127-36
公開日 2014-2-1
DOI 10.1016/j.mod.2013.09.003
PII S0925-4773(13)00079-8
PMID 24157522
MeSH Animals Cell Differentiation / genetics* Embryo, Mammalian / metabolism Endoderm / growth & development Endoderm / metabolism Gastrointestinal Tract / growth & development* Gastrointestinal Tract / metabolism Gene Expression Regulation, Developmental Hedgehog Proteins / biosynthesis* Hedgehog Proteins / genetics Lung / growth & development* Lung / metabolism Mesoderm / growth & development Mesoderm / metabolism Mice Morphogenesis Regulatory Elements, Transcriptional / genetics* Signal Transduction
IF 2.126
引用数 21
リソース情報
遺伝子材料 mouse MACS1-luc2 (RDB17896) spret MACS1-luc2 (RDB17897) rat MACS1-luc2 (RDB17898) rabbit MACS1-luc2 (RDB17899) mouse SLGE-luc2 (RDB17900) spret SLGE-luc2 (RDB17901) rat SLGE-luc2 (RDB17902) rabbit SLGE-luc2 (RDB17903)