論文 - 詳細
| RRC ID | 58322 |
|---|---|
| 著者 | Arasaki K, Nagashima H, Kurosawa Y, Kimura H, Nishida N, Dohmae N, Yamamoto A, Yanagi S, Wakana Y, Inoue H, Tagaya M. |
| タイトル | MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules. |
| ジャーナル | EMBO Rep |
| Abstract |
In fed cells, syntaxin 17 (Stx17) is associated with microtubules at the endoplasmic reticulum-mitochondria interface and promotes mitochondrial fission by determining the localization and function of the mitochondrial fission factor Drp1. Upon starvation, Stx17 dissociates from microtubules and Drp1, and binds to Atg14L, a subunit of the phosphatidylinositol 3-kinase complex, to facilitate phosphatidylinositol 3-phosphate production and thereby autophagosome formation, but the mechanism underlying this phenomenon remains unknown. Here we identify MAP1B-LC1 (microtubule-associated protein 1B-light chain 1) as a critical regulator of Stx17 function. Depletion of MAP1B-LC1 causes Stx17-dependent autophagosome accumulation even under nutrient-rich conditions, whereas its overexpression blocks starvation-induced autophagosome formation. MAP1B-LC1 links microtubules and Stx17 in fed cells, and starvation causes the dephosphorylation of MAP1B-LC1 at Thr217, allowing Stx17 to dissociate from MAP1B-LC1 and bind to Atg14L. Our results reveal the mechanism by which Stx17 changes its binding partners in response to nutrient status. |
| 巻・号 | 19(8) |
| 公開日 | 2018-8-1 |
| DOI | 10.15252/embr.201745584 |
| PII | embr.201745584 |
| PMID | 29925525 |
| PMC | PMC6073212 |
| MeSH | Autophagosomes / metabolism* Autophagy Endoplasmic Reticulum / metabolism Gene Knockdown Techniques HeLa Cells Humans Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Mitochondria / metabolism Phosphatidylinositol Phosphates / metabolism Phosphorylation Phosphothreonine / metabolism Protein Binding Qa-SNARE Proteins / metabolism* Tubulin / metabolism |
| IF | 7.497 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | FLAG-MAP1B-LC1 1-243 (RDB17877) FLAG-MAP1B-LC1 1-218 (RDB17878) FLAG-MAP1B-LC1 1-207 (RDB17879) FLAG-MAP1B-LC1 T217A (RDB17880) FLAG-MAP1B-LC1 T217E (RDB17881) |
| ヒト・動物細胞 | HeLa(RCB0007) |