RRC ID 58329
Author Megha, Wegener C, Hasan G.
Title ER-Ca2+ sensor STIM regulates neuropeptides required for development under nutrient restriction in Drosophila.
Journal PLoS One
Abstract Neuroendocrine cells communicate via neuropeptides to regulate behaviour and physiology. This study examines how STIM (Stromal Interacting Molecule), an ER-Ca2+ sensor required for Store-operated Ca2+ entry, regulates neuropeptides required for Drosophila development under nutrient restriction (NR). We find two STIM-regulated peptides, Corazonin and short Neuropeptide F, to be required for NR larvae to complete development. Further, a set of secretory DLP (Dorso lateral peptidergic) neurons which co-express both peptides was identified. Partial loss of dSTIM caused peptide accumulation in the DLPs, and reduced systemic Corazonin signalling. Upon NR, larval development correlated with increased peptide levels in the DLPs, which failed to occur when dSTIM was reduced. Comparison of systemic and cellular phenotypes associated with reduced dSTIM, with other cellular perturbations, along with genetic rescue experiments, suggested that dSTIM primarily compromises neuroendocrine function by interfering with neuropeptide release. Under chronic stimulation, dSTIM also appears to regulate neuropeptide synthesis.
Volume 14(7)
Pages e0219719
Published 2019-1-1
DOI 10.1371/journal.pone.0219719
PII PONE-D-19-17468
PMID 31295329
PMC PMC6622525
MeSH Animals Calcium / metabolism Calcium Signaling / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics Gene Expression Regulation / genetics Larva / genetics Larva / growth & development Neurons / metabolism* Neuropeptides / genetics* Neurosecretory Systems Nutrients / metabolism Pupa / genetics Pupa / growth & development RNA, Messenger / genetics Stromal Interaction Molecule 1 / genetics*
IF 2.74
Times Cited 3
Drosophila DGRC#113901