| Abstract |
To better understand the promotion effect of phytic acid and its uptake mechanism in freshwater microalga Euglena gracilis, cell growth, photosynthetic pigment content and cell morphology of E. gracilis were evaluated under four conditions: phosphorus deficient group (CMP-), single phosphate treatment group (CMP+), single phytic acid treatment group (CMPA-), and phosphate-phytic acid mixed treatment group (CMPA+). The results showed that phytic acid could serve as the sole phosphorus source for the growth of E. gracilis, and phytase which catalyzes the hydrolysis of phytic acid was discovered for the first time in E. gracilis. Fourier transform infrared spectroscopy combined with multivariate analysis showed the good recognition of metabolites from different culture conditions especially focusing on relative carbohydrate or lipid contents. Phytic acid derived from agro-wastes is a cheap growth promoter for E. gracilis, and this E. gracilis with high nutritional value is applicable to animal feed while minimizing environmental impact.
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