Reference - Detail
| RRC ID | 58363 |
|---|---|
| Author | Yamano T, Fukuzawa H. |
| Title | Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. |
| Journal | Methods Mol Biol |
| Abstract |
The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions. |
| Volume | 2050 |
| Pages | 155-161 |
| Published | 2020-1-1 |
| DOI | 10.1007/978-1-4939-9740-4_16 |
| PMID | 31468489 |
| MeSH | Cell Wall Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / growth & development Electroporation / instrumentation* Equipment Design Transfection / instrumentation* Transformation, Genetic |
| Times Cited | 1 |
| Resource | |
| Algae | NIES-2235 |